PMID: 2119503Sep 1, 1990Paper

Conservation of infectivity in purified fibrillary extracts of scrapie-infected hamster brain after sequential enzymatic digestion or polyacrylamide gel electrophoresis

Proceedings of the National Academy of Sciences of the United States of America
P BrownD C Gajdusek

Abstract

Infectious extracts of scrapie-infected hamster brain enriched for scrapie-associated fibrils and scrapie amyloid protein (PrP) were partially denatured and subjected to either polyacrylamide gel electrophoresis with subsequent isolation of the PrP band or sequential enzymatic digestion with deglycosidase, phospholipase, proteinase, and several different nucleases. Infectivity measurements of these various specimens revealed a convincing association between infectivity and scrapie amyloid protein, with or without its sugar chains and disulfide bonds, and did not support the hypothesis that nucleic acid is involved in replication.

References

Aug 1, 1976·The Journal of General Virology·G D HunterR H Kimberlin
Aug 11, 1990·Proceedings of the National Academy of Sciences of the United States of America·J SafarC J Gibbs
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J R DuguidB Seed
Apr 1, 1985·The Journal of General Virology·T L GermanR F Marsh
Nov 1, 1987·Archives of Biochemistry and Biophysics·D C BoltonA Potempska
Dec 1, 1988·The Journal of Infectious Diseases·P E BendheimD C Bolton
Aug 15, 1986·Cell·G A CarlsonS B Prusiner
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T SklaviadisE E Manuelidis
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R GabizonS B Prusiner
May 20, 1967·Nature·T AlperM C Clarke
Jul 1, 1967·Journal of Comparative Pathology·G D Hunter, G C Millson
Aug 1, 1972·Journal of Neurochemistry·D H Adams
Feb 1, 1982·The Journal of General Virology·M T BorrásC J Gibbs
Dec 1, 1983·Cell·S B PrusinerG G Glenner
Dec 24, 1982·Science·D C BoltonS B Prusiner

❮ Previous
Next ❯

Citations

Jan 1, 1996·Archives of Virology·R A Somerville, A J Dunn
Jan 11, 1991·Brain Research. Brain Research Reviews·D C GajdusekK K Hsiao
May 1, 1992·Virus Research·K SasakiM Shinagawa
Sep 9, 2005·Nature·Jay R SilveiraByron Caughey
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·S B PrusinerR Gabizon
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S ZhangA Rich
May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·L ManuelidisW Fritch
Apr 17, 2009·The Journal of Biological Chemistry·Laszlo L P HosszuAnthony R Clarke
Jun 1, 1991·New Zealand Veterinary Journal·P R Wills
Jun 6, 1994·Annals of the New York Academy of Sciences·J Hope
Jan 16, 2016·International Journal of Molecular Sciences·Abigail B DiackJean C Manson
Nov 4, 2008·Journal of Neuroimmunology·Binggong ChangRichard Rubenstein
Jan 8, 2009·International Journal of Dermatology·Michelle Gralle Botelho, Omar Lupi
Aug 22, 1991·Nature·C Weissmann
Apr 1, 1991·The British Journal of Psychiatry : the Journal of Mental Science·P J Harrison, G W Roberts
May 1, 1995·Histopathology·M BarcikowskaD C Gajdusek
May 17, 2000·The Journal of General Virology·T ManousisT K Sklaviadis
Oct 1, 1994·Transfusion·L Manuelidis
Apr 22, 1994·Science·F E CohenS B Prusiner

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.