Conservation of protein kinase a catalytic subunit sequences in the schistosome pathogens of humans.

Experimental Parasitology
Brett E Swierczewski, S J Davies

Abstract

cAMP-dependent protein kinases (PKAs) are central mediators of cAMP signaling in eukaryotic cells. Previously we identified a cDNA which encodes for a PKA catalytic subunit (PKA-C) in Schistosoma mansoni (SmPKA-C) that is required for adult schistosome viability in vitro. As such, SmPKA-C could potentially represent a novel schistosome chemotherapeutic target. Here we sought to identify PKA-C subunit orthologues in the other medically important schistosome species, Schistosoma haematobium and Schistosoma japonicum, to determine the degree to which this potential target is conserved and could therefore be exploited for the treatment of all forms of schistosomiasis. We report the identification of PKA-C subunit orthologues in S. haematobium and S. japonicum (ShPKA-C and SjPKA-C, respectively) and show that PKA-C orthologues are highly conserved in the Schistosoma, with over 99% amino acid sequence identity shared among the three human pathogens we examined. Furthermore, we show that the recently published Schistosoma mansoni and S. japonicum genomes contain sequences encoding for several putative PKA substrates with homology to those found in Homo sapiens, Caenorhabditis elegans, and Saccharomyces cerevisiae.

References

Mar 1, 1995·Molecular Phylogenetics and Evolution·A Zharkikh, W H Li
Jul 1, 1994·The American Journal of Tropical Medicine and Hygiene·P G Fallon, M J Doenhoff
Jun 29, 1993·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·S S TaylorW M Yonemoto
Jun 18, 2003·Parasitology Research·Donato Cioli, Livia Pica-Mattoccia
Jan 1, 2004·Molecular and Cellular Biology·Heehyoung LeeEdward Seto
May 30, 2006·International Journal for Parasitology·Bonnie L WebsterD Timothy J Littlewood
Sep 26, 2006·Lancet·Bruno GryseelsLuc Kestens
Dec 22, 2006·Proceedings of the National Academy of Sciences of the United States of America·Rahul DasGiuseppe Melacini
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Jul 27, 2007·Current Opinion in Chemical Biology·Conor R Caffrey
Nov 21, 2007·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Colette DissousNaji Khayath
Aug 13, 2008·Infection and Immunity·Yi BaoHuan Huang
Nov 4, 2008·Current Opinion in Infectious Diseases·Michael J DoenhoffJürg Utzinger
Aug 19, 2009·PLoS Neglected Tropical Diseases·Sandra D MelmanEric S Loker
Aug 27, 2009·PLoS Neglected Tropical Diseases·Brett E Swierczewski, Stephen J Davies

❮ Previous
Next ❯

Citations

Jan 19, 2016·International Journal for Parasitology·Natasha L HirstAnthony J Walker
May 25, 2010·International Journal for Parasitology·T QuackC G Grevelding

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Anthelmintics (ASM)

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Related Papers

International Journal for Parasitology
Jaap J Van HellemondAloysius G M Tielens
BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology
Colette DissousNaji Khayath
© 2021 Meta ULC. All rights reserved