Conservation of the 2,4-diacetylphloroglucinol biosynthesis locus among fluorescent Pseudomonas strains from diverse geographic locations.

Applied and Environmental Microbiology
Christoph KeelLinda S Thomashow

Abstract

The broad-spectrum antibiotic 2,4-diacetylphloroglucinol (PHL) is a major determinant in the biological control of a range of plant pathogens by many fluorescent Pseudomonas spp. A 4.8-kb chromosomal DNA region from Pseudomonas fluorescens Q2-87, carrying PHL biosynthetic genes, was used as a probe to determine if the PHL biosynthetic locus is conserved within PHL-producing Pseudomonas strains of worldwide origin. The phl gene probe hybridized with the genomic DNA of all 45 PHL-producing Pseudomonas strains tested, including well-characterized biocontrol strains from the United States and Europe and strains isolated from disease-suppressive soils from Switzerland, Washington, Italy, and Ghana. The PHL producers displayed considerable phenotypic and genotypic diversity. Two phenotypically distinct groups were detected. The first produced PHL, pyoluteorin, and hydrogen cyanide and consisted of 13 strains from almost all locations sampled in the United States, Europe, and Africa. The second produced only PHL and HCN and consisted of 32 strains from the U.S. and European soils. Analysis of restriction patterns of genomic DNA obtained after hybridization with the phl gene probe and cluster analysis of restriction patterns of amplifi...Continue Reading

References

Jan 1, 1979·Methods in Enzymology·F Bolivar, K Backman
Aug 20, 1992·Journal of Molecular Biology·M GamperD Haas
Oct 1, 1991·Applied and Environmental Microbiology·M N VincentE A Pierson
Nov 25, 1990·Nucleic Acids Research·J G WilliamsS V Tingey
Feb 1, 1972·Genetical Research·V A Stanisich, B W Holloway
Sep 1, 1969·Bacteriological Reviews·B W Holloway
Dec 1, 1994·Applied and Environmental Microbiology·E V StabbJ Handelsman
Oct 1, 1994·Plant Molecular Biology·B KollerC Gessler
Jan 1, 1985·Applied and Environmental Microbiology·W D GouldR M Zablotowicz

❮ Previous
Next ❯

Citations

Nov 9, 2002·Molecular Plant-microbe Interactions : MPMI·Henk-jan SchoonbeekMaarten A De Waard
May 6, 2003·Annual Review of Phytopathology·Brion DuffyJos M Raaijmakers
Apr 7, 2004·Applied and Environmental Microbiology·Monika MaurhoferChristoph Keel
Oct 24, 2008·Phytopathology·Brian B McSpadden GardenerElizabeth Lutton
Feb 3, 2009·Applied and Environmental Microbiology·Jennifer A MoynihanE Fidelma Boyd
Jun 16, 2011·Journal of Applied Microbiology·N ClermontC Beaulieu
Dec 6, 2012·Applied Microbiology and Biotechnology·Hianna Almeida Câmara LeiteLeandro Lopes Loguercio
Dec 19, 2016·The Journal of General and Applied Microbiology·Hayron CanchigniaHumberto Prieto
Mar 11, 2020·Scientific Reports·Ming-Min ZhaoHong-You Zhou
Nov 10, 2001·Annual Review of Phytopathology·CL BenderJ Loper
Jul 31, 2002·Annual Review of Phytopathology·David M WellerLinda S Thomashow
May 2, 2000·Applied and Environmental Microbiology·B B McSpadden GardenerD M Weller
Mar 24, 2005·Molecular Plant-microbe Interactions : MPMI·Maria Péchy-TarrChristoph Keel
Feb 7, 2003·Applied and Environmental Microbiology·Sandrine DelormePhilippe Lemanceau
Oct 22, 2009·Natural Product Reports·Harald Gross, Joyce E Loper

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.