Conservation of the genetic switch between replication and transfer genes of IncP plasmids but divergence of the replication functions which are major host-range determinants

Plasmid
P B ThorstedC M Thomas

Abstract

The trfA operon of broad-host-range IncP plasmids is essential to activate the origin of vegetative replication in diverse species. The trb operon encodes most of the apparatus for mating pair formation, the first step in conjugative transfer. Comparison of the nucleotide sequence of the IncP beta plasmid R751 presented here with the equivalent IncP alpha sequence identifies conserved features of the organization and regulation of the trfA operon and the region controlling expression of the trb operon. As in IncP alpha plasmids, these operons are transcribed from a bidirectional promoter region consisting of trfAp for the trfA operon and trbAp and trbBp for the trb operon. The KorA-dependent switch between the trfA and trbA promoters is conserved as is the trbA gene encoding the third IncP global regulator. The intergenic region between trbA and trbB shows very little sequence identity between the two plasmids but the spacing, the KorB operator, the trbB promoter, and the existence of a hairpin loop (albeit of different actual sequence) which sequesters the trbB ribosome binding site are all conserved. The trfA operon encodes two ORFs. The first ORF is highly conserved and encodes a putative single-stranded DNA binding protein ...Continue Reading

Citations

May 13, 1999·Molecular Microbiology·G Jagura-BurdzyC M Thomas
Mar 14, 2012·Proceedings of the National Academy of Sciences of the United States of America·Zhigang QiuJun-Wen Li
May 27, 2008·Applied and Environmental Microbiology·Anna KulinskaGrazyna Jagura-Burdzy
Jan 10, 2012·Journal of Bacteriology·Hirokazu YanoEva M Top
Feb 24, 2001·Journal of Bacteriology·M ZatykaC M Thomas
Nov 9, 2011·Environmental Microbiology·Yingfei MaBrian Palenik
May 14, 2005·Journal of Molecular Biology·Lewis E H BingleChristopher M Thomas
Jul 7, 2020·Frontiers in Microbiology·Masatoshi MiyakoshiMasataka Tsuda
Jun 14, 2020·Applied and Environmental Microbiology·Ewa LewickaGrazyna Jagura-Burdzy
Mar 7, 2020·Frontiers in Microbiology·Johannes WernerBaşak Öztürk
May 29, 2002·Biochimica Et Biophysica Acta·Kalliopi Kostelidou, Christopher M Thomas

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