Conservation of the Pho regulon in Pseudomonas fluorescens Pf0-1

Applied and Environmental Microbiology
Russell D MondsGeorge A O'Toole

Abstract

The Pho regulon integrates the sensing of environmental inorganic phosphate (Pi) availability with coregulation of gene expression, mediating an adaptive response to Pi limitation. Many aspects of the Pho regulon have been addressed in studies of Escherichia coli; however, it is unclear how transferable this knowledge is to other bacterial systems. Here, we report work to discern the conservation of the Pho regulon in Pseudomonas fluorescens Pf0-1. We demonstrate by mutational studies that PhoB/PhoR and the Pst system have conserved functions in the regulation of Pi-induced phosphatase activities, as well as expression of other Pi-regulated genes. A genetic screen was carried out to isolate factors that affect Pho-regulated phosphatase activity. We identified the Pho-regulated phosphatases PhoX and PhoD and present evidence that these enzymes are exported via the Tat system. The phoX and phoD genes were shown to be members of the Pho regulon by reverse transcription-PCR, as well as by functional assessment of putative PhoB binding sites (Pho boxes). Our data also suggested that at least one other non-Tat-secreted Pho-regulated phosphatase exists. From the genetic screen, numerous siderophore mutants that displayed severe defect...Continue Reading

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Citations

Feb 17, 2009·Proceedings of the National Academy of Sciences of the United States of America·Peter D NewellGeorge A O'Toole
Apr 17, 2009·Proceedings of the National Academy of Sciences of the United States of America·Alexander ZaborinJohn C Alverdy
Apr 12, 2012·Applied and Environmental Microbiology·Mark W SilbyStuart B Levy
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Feb 20, 2008·PLoS Pathogens·Olga ZaborinaJohn C Alverdy
Mar 26, 2016·Frontiers in Microbiology·Alexander Crits-ChristophJocelyne DiRuggiero
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