Conservation of Transit Peptide-Independent Protein Import into the Mitochondrial and Hydrogenosomal Matrix

Genome Biology and Evolution
Sriram GargSven B Gould

Abstract

The origin of protein import was a key step in the endosymbiotic acquisition of mitochondria. Though the main translocon of the mitochondrial outer membrane, TOM40, is ubiquitous among organelles of mitochondrial ancestry, the transit peptides, or N-terminal targeting sequences (NTSs), recognised by the TOM complex, are not. To better understand the nature of evolutionary conservation in mitochondrial protein import, we investigated the targeting behavior of Trichomonas vaginalis hydrogenosomal proteins in Saccharomyces cerevisiae and vice versa. Hydrogenosomes import yeast mitochondrial proteins even in the absence of their native NTSs, but do not import yeast cytosolic proteins. Conversely, yeast mitochondria import hydrogenosomal proteins with and without their short NTSs. Conservation of an NTS-independent mitochondrial import route from excavates to opisthokonts indicates its presence in the eukaryote common ancestor. Mitochondrial protein import is known to entail electrophoresis of positively charged NTSs across the electrochemical gradient of the inner mitochondrial membrane. Our present findings indicate that mitochondrial transit peptides, which readily arise from random sequences, were initially selected as a signal ...Continue Reading

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Citations

Feb 14, 2016·Genome Biology and Evolution·Tereza ŠevčíkováMarek Eliáš
Jun 28, 2016·Genome Biology and Evolution·Sriram G Garg, William F Martin
Aug 16, 2016·Trends in Cell Biology·Sriram G Garg, Sven B Gould
Jun 22, 2017·Genome Biology and Evolution·Martina BradicJane M Carlton
Apr 7, 2018·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Priscila Peña-Diaz, Julius Lukeš
Jun 16, 2017·Microbiology and Molecular Biology Reviews : MMBR·William F MartinSven B Gould
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Sep 29, 2018·Genome Biology and Evolution·Joana Pereira, Andrei N Lupas
Apr 4, 2017·Molecular Biology and Evolution·Yoshinori FukasawaKenichiro Imai
Jul 31, 2018·Genome Biology and Evolution·Carolina Río BártulosMark van der Giezen
Sep 12, 2018·Trends in Parasitology·Herbert J SantosTomoyoshi Nozaki

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Methods Mentioned

BETA
proteomic profiling
electrophoresis
confocal microscopy
transfection
GTPase

Software Mentioned

Proteome Discoverer
TargetP
pepstats
EMBOSS package
ImageJ
Mascot
MaxQuant
TrichDB

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