Conserved amino acid sequences confer nuclear localization upon the Prophet of Pit-1 pituitary transcription factor protein

Gene
J Chico GuyS J Rhodes

Abstract

Prophet of Pit-1 (PROP1) is a homeodomain transcription factor essential for development of the mammalian anterior pituitary gland. Studies of human patients and animal models with mutations in their Prop1 genes have established that PROP1 is required for the correct development or sustained function of the hormone-secreting cells that regulate physiological pathways controlling growth, reproduction, metabolism, and the stress response. By comparative analysis of mammalian Prop1 genes and their encoded proteins, including cloning the ovine Prop1 gene and its products, we demonstrate that two conserved basic regions (B1 and B2) of the PROP1 protein located within the homeodomain are required for nuclear localization, DNA binding, and target gene activation. Interestingly, missense mutations in the human Prop1 gene causing amino acid changes in both the B1 and B2 regions have been associated with combined pituitary hormone deficiency (CPHD) diseases, suggesting that disruption of nuclear localization may be part of the molecular basis of such diseases. The ovine Prop1 gene has three exons and two introns, a different structure compared with that of the bovine gene. Two alleles of the ovine gene were found to encode protein produc...Continue Reading

References

Feb 11, 1988·Nucleic Acids Research·S A MillerH F Polesky
Feb 14, 1998·Nature Genetics·W WuM G Rosenfeld
Feb 18, 1999·Journal of Cellular Biochemistry·M G ManciniM A Mancini
Jun 8, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D A JansM H Lam
Nov 7, 2000·Molecular and Cellular Endocrinology·K W SloopS J Rhodes
Mar 22, 2001·EMBO Reports·M CokolB Rost
Sep 28, 2001·Genome Biology·S A Adam
Mar 22, 2002·Molecular and Cellular Neurosciences·Y Alex ZhangSusan K McConnell
Apr 17, 2002·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Roger T StoneGary L Bennett
Aug 14, 2002·Annals of Medicine·Lisa J CushmanSimon J Rhodes
Oct 23, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Keizo FurukawaTomoo Tsukazaki
Dec 5, 2002·Best Practice & Research. Clinical Endocrinology & Metabolism·Sushil ModyJohn S Parks
Mar 26, 2003·Genes & Development·Catherine E Keegan, Sally A Camper
Jun 11, 2003·Recent Progress in Hormone Research·Lorin E OlsonMichael G Rosenfeld
Sep 6, 2003·Gene·Marta AlvarezJoseph P Bidwell

❮ Previous
Next ❯

Citations

Jun 9, 2007·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Robert D WardSally A Camper
Mar 15, 2011·Best Practice & Research. Clinical Endocrinology & Metabolism·R Pfäffle, J Klammt
Jun 9, 2005·The Journal of Clinical Endocrinology and Metabolism·Rachel ReynaudThierry Brue
Nov 26, 2010·Molecular Biology Reports·Xian-Cun ZengYing-Chun Du

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.