Conserved central domains control the quaternary structure of type I and type II Hsp40 molecular chaperones.

Journal of Molecular Biology
Carlos H I RamosDouglas M Cyr

Abstract

Heat shock protein (Hsp)40s play an essential role in protein metabolism by regulating the polypeptide binding and release cycle of Hsp70. The Hsp40 family is large, and specialized family members direct Hsp70 to perform highly specific tasks. Type I and Type II Hsp40s, such as yeast Ydj1 and Sis1, are homodimers that dictate functions of cytosolic Hsp70, but how they do so is unclear. Type I Hsp40s contain a conserved, centrally located cysteine-rich domain that is replaced by a glycine- and methionine-rich region in Type II Hsp40s, but the mechanism by which these unique domains influence Hsp40 structure and function is unknown. This is the case because high-resolution structures of full-length forms of these Hsp40s have not been solved. To fill this void, we built low-resolution models of the quaternary structure of Ydj1 and Sis1 with information obtained from biophysical measurements of protein shape, small-angle X-ray scattering, and ab initio protein modeling. Low-resolution models were also calculated for the chimeric Hsp40s YSY and SYS, in which the central domains of Ydj1 and Sis1 were exchanged. Similar to their human homologs, Ydj1 and Sis1 each has a unique shape with major structural differences apparently being th...Continue Reading

References

Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·K LiberekM Zylicz
Aug 1, 1991·The Journal of Cell Biology·A J Caplan, M G Douglas
Dec 15, 1995·Journal of Molecular Biology·S DoniachH Orland
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·A SzaboF U Hartl
Apr 1, 1994·Trends in Biochemical Sciences·D M CyrM G Douglas
Aug 9, 1996·The Journal of Biological Chemistry·Y MinamiF U Hartl
May 19, 1998·Cell Stress & Chaperones·M E Cheetham, A J Caplan
Mar 17, 1999·The EMBO Journal·G C MeachamD M Cyr
Mar 11, 2000·Molecular Cell·N Sondheimer, S Lindquist
Jul 13, 2000·Journal of Molecular Biology·M Martinez-YamoutH J Dyson
Jan 12, 2002·Current Opinion in Structural Biology·T Laue
Mar 9, 2002·Science·F Ulrich Hartl, Manajit Hayer-Hartl
Mar 13, 2003·Molecular Biology of the Cell·Nelson LopezElizabeth A Craig
Dec 6, 2003·Molecular Biology of the Cell·Chun-Yang FanDouglas M Cyr
Apr 30, 2004·Cell Stress & Chaperones·Chun-Yang FanDouglas M Cyr
Mar 22, 2005·Protein and Peptide Letters·Júlio C Borges, Carlos H I Ramos
Jun 1, 2005·Biophysical Journal·Maxim V Petoukhov, Dmitri I Svergun
Aug 18, 2005·Proceedings of the National Academy of Sciences of the United States of America·Patrick R D'SilvaElizabeth A Craig
Apr 4, 2006·Archives of Biochemistry and Biophysics·Cristiano L P OliveiraCarlos H I Ramos
Feb 1, 2007·Annual Review of Biochemistry·Walter Neupert, Johannes M Herrmann
Mar 6, 2007·Journal of Bacteriology·Matthew R ChenowethSue Wickner
Jan 1, 2004·Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology·Carlos Henrique I Ramos

❮ Previous
Next ❯

Citations

Jun 25, 2009·Proceedings of the National Academy of Sciences of the United States of America·Pradeep KotaNikolay V Dokholyan
Aug 24, 2010·The Journal of Biological Chemistry·Judit Perales-CalvoFernando Moro
Oct 24, 2012·The Journal of Biological Chemistry·Imad BaakliniJason C Young
Dec 6, 2008·The Journal of Biological Chemistry·Daniel W SummersDouglas M Cyr
May 11, 2010·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Jason C Young
Apr 11, 2009·Trends in Biochemical Sciences·Daniel W SummersDouglas M Cyr
Jun 19, 2009·Prion·Daniel W SummersDouglas M Cyr
Aug 8, 2015·Nature·Nadinath B NillegodaBernd Bukau
Sep 12, 2019·Protein Science : a Publication of the Protein Society·Qinglian LiuLei Zhou
May 2, 2018·Biomolecular NMR Assignments·Glaucia M S PinheiroFabio C L Almeida
May 17, 2017·Biophysics Reviews·Júlio C BorgesLeandro R S Barbosa
Nov 3, 2021·Scientific Reports·Lukas SchmauderKlaus Richter
Dec 8, 2021·Proceedings of the National Academy of Sciences of the United States of America·Hubert WyszkowskiKrzysztof Liberek
Jan 3, 2012·International Journal of Biological Macromolecules·Maria J FiguerasSergio O Angel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.