Conserved cytoplasmic domain sequences mediate the ER export of VZV, HSV-1, and HCMV gB

Virology
T C HeinemanDaniel Assefa

Abstract

Glycoprotein B (gB) is conserved among the herpesviruses and participates in both virus entry and cell-cell spread. The ER export of VZV gB is mediated by two cytoplasmic domain regions, aa 818-826, which contains a YXXphi motif, and the C-terminal 17 aa. The current study examines whether related sequences in the cytoplasmic domains of HSV-1 and HCMV gB similarly influence the ER export of their gB homologs. Directed mutations were introduced into the cytoplasmic domains of HSV-1 and HCMV gB, and the efficiencies with which the mutated proteins acquired Golgi-dependent modifications were determined. Sequences homologous to VZV gB aa 818-826 were required for normal ER export of both HSV-1 gB and HCMV gB. However, the C-terminal regions of HSV-1 and HCMV gB had no impact on ER export. Therefore, alpha- and betaherpesvirus gB homologs share conserved ER export signals, but species-specific differences in the ER export of gB also exist.

References

Nov 1, 1990·Nature·B MossT R Fuerst
Jan 1, 1989·Annual Review of Cell Biology·S M Hurtley, A Helenius
Sep 1, 1986·The Journal of General Virology·A J Davison, J E Scott
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T R FuerstB Moss
Jul 1, 1996·The Journal of General Virology·G S MarshallL A Hunt
Jul 1, 1997·The Journal of General Virology·E BognerK Radsak
May 16, 1998·The Journal of Cell Biology·M AridorW E Balch
May 30, 1998·Journal of Virology·R S Tirabassi, L W Enquist
Aug 8, 1998·Trends in Cell Biology·S I BannykhW E Balch
Dec 16, 1998·The Journal of Infectious Diseases·Z WangA A Gershon
Mar 12, 1999·Journal of Virology·R S Tirabassi, L W Enquist
Mar 30, 2001·The Journal of Biological Chemistry·M M ZareiL Toro
Dec 26, 2001·Journal of Virology·Thomas C Heineman, Susan L Hall
Jan 19, 2002·Journal of Virology·Thomas C Mettenleiter

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Citations

Jan 6, 2009·Immunological Reviews·Shin-Ichiroh Saitoh, Kensuke Miyake
Jan 19, 2006·Virus Research·Herman W Favoreel
Jan 26, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Naima NiazyNorbert Koch

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