Conserved domains subserve novel mechanisms and functions in DKF-1, a Caenorhabditis elegans protein kinase D

The Journal of Biological Chemistry
Hui FengC S Rubin

Abstract

Protein kinase D (PKD) isoforms are effectors in signaling pathways controlled by diacylglycerol. PKDs contain conserved diacylglycerol binding (C1a, C1b), pleckstrin homology (PH), and Ser/Thr kinase domains. However, the properties of conserved domains may vary within the context of distinct PKD polypeptides. Such functional/structural malleability (plasticity) was explored by studying Caenorhabditis elegans D kinase family-1 (DKF-1), a PKD that governs locomotion in vivo. Phorbol ester binding with C1b alone activates classical PKDs by relieving C1-mediated inhibition. In contrast, C1a avidly ligated phorbol 12-myristate 13-acetate (PMA) and anchored DKF-1 at the plasma membrane. C1b bound PMA (moderate affinity) and cooperated with C1a in targeting DKF-1 to membranes. Mutations at a "Pro(11)" position in C1 domains were inactivating; kinase activity was minimal at PMA concentrations that stimulated wild type DKF-1 approximately 10-fold. DKF-1 mutants exhibited unchanged, maximum kinase activity after cells were incubated with high PMA concentrations. Titration in situ revealed that translocation and activation of wild type and mutant DKF-1 were tightly and quantitatively linked at all PMA concentrations. Thus, C1 domains po...Continue Reading

References

Aug 30, 1994·Proceedings of the National Academy of Sciences of the United States of America·A M ValverdeE Rozengurt
Sep 1, 1995·Medicinal Research Reviews·G S KroogJ F Battey
Jan 10, 1997·The Journal of Biological Chemistry·K NishikawaL C Cantley
Feb 7, 1998·The Journal of Biological Chemistry·T Iglesias, E Rozengurt
Feb 3, 1998·Molecular and Cellular Biology·Z LuD A Foster
Oct 9, 1998·The Journal of Biological Chemistry·T IglesiasE Rozengurt
May 8, 1999·Biochimica Et Biophysica Acta·A HayashiT Saito
Jul 21, 1999·FEBS Letters·T Iglesias, E Rozengurt
Jun 17, 2000·The EMBO Journal·S A MatthewsD Cantrell
Aug 6, 2000·The Biochemical Journal·M A Lemmon, K M Ferguson
Aug 15, 2000·Annual Review of Biophysics and Biomolecular Structure·J H Hurley, S Misra
Sep 22, 2000·The Journal of Biological Chemistry·T IglesiasG Schiavo
Jun 19, 2001·The Journal of Biological Chemistry·R T WaldronE Rozengurt
Sep 11, 2001·Biochemical and Biophysical Research Communications·O Rey, E Rozengurt
Dec 14, 2001·The Journal of Biological Chemistry·Ilona BrändlinFranz-Josef Johannes
Jan 5, 2002·The Journal of Cell Biology·Angelika HausserFranz-Josef Johannes
Jan 11, 2002·Molecular and Cellular Biology·Ying WangJohn Colicelli
Apr 30, 2002·Trends in Cell Biology·Johan Van LintThomas Seufferlein
Sep 5, 2002·The Biochemical Journal·Jyoti SrivastavaPeter J Parker
Dec 10, 2002·Science·G ManningS Sudarsanam
Mar 15, 2003·The Journal of Biological Chemistry·Kent L RossmanJohn Sondek
Mar 15, 2003·The Journal of Biological Chemistry·Peter StorzAlex Toker
Apr 12, 2003·Developmental Cell·Elena OanceaDavid E Clapham
Jun 6, 2003·Current Opinion in Immunology·Doreen A Cantrell
Jun 28, 2003·FEBS Letters·An RykxJohan Van Lint
Jun 24, 2004·Seminars in Cell & Developmental Biology·Alfonso MoraDario R Alessi

❮ Previous
Next ❯

Citations

Nov 26, 2008·The Journal of Biological Chemistry·Vitalyi O RybinSusan F Steinberg
Apr 29, 2008·BMC Developmental Biology·Kornelia EllwangerAngelika Hausser
Jan 5, 2013·IUBMB Life·Kornelia Ellwanger, Angelika Hausser
Jan 31, 2020·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Ronja ReinhardtThomas A Leonard
Feb 6, 2017·The Biochemical Journal·Rossana ZaruUNKNOWN UniProt Consortium

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.