Jul 1, 1990

Conserved enhancer and silencer elements responsible for differential Adh transcription in Drosophila cell lines

Molecular and Cellular Biology
S Ayer, C Benyajati

Abstract

The distal promoter of Adh is differentially expressed in Drosophila tissue culture cell lines. After transfection with an exogenous Adh gene, there was a specific increase in distal alcohol dehydrogenase (ADH) transcripts in ADH-expressing (ADH+) cells above the levels observed in transfected ADH-nonexpressing (ADH-) cells. We used deletion mutations and a comparative transient-expression assay to identify the cis-acting elements responsible for enhanced Adh distal transcription in ADH+ cells. DNA sequences controlling high levels of distal transcription were localized to a 15-base-pair (bp) region nearly 500 bp upstream of the distal RNA start site. In addition, a 61-bp negative cis-acting element was found upstream from and adjacent to the enhancer. When this silencer element was deleted, distal transcription increased only in the ADH+ cell line. These distant upstream elements must interact with the promoter elements, the Adf-1-binding site and the TATA box, as they only influenced transcription when at least one of these two positive distal promoter elements was present. Internal deletions targeted to the Adf-1-binding site or the TATA box reduced transcription in both cell types but did not affect the transcription initia...Continue Reading

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Mentioned in this Paper

Transfection
Transcription, Genetic
Alcoholic Intoxication, Chronic
Drosophila
Adf1
Genes, Regulator
Alcohol Dehydrogenase II
Cistron
Drosophila melanogaster
Nucleotide Mapping

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