Conserved expression of the Arabidopsis ACT1 and ACT 3 actin subclass in organ primordia and mature pollen

The Plant Cell
Y Q AnR B Meagher

Abstract

We have proposed that ancient and divergent classes of plant actin genes have been preserved throughout vascular plant evolution, because they have distinct patterns of gene regulation. The hypothesis was explored for ACT1 and ACT3, which represent one of the six ancient subclasses in the Arabidopsis actin gene family. Comparison of ACT1 and ACT3 cDNA and genomic sequences revealed highly divergent flaking and intron sequences, whereas they encoded nearly identical proteins. Quantification of their level of divergence suggests that they have not shared a common ancestor for 30 to 60 million years. Gene-specific RNA gel blot hybridization and reverse transcriptase-polymerase chain reaction analyses demonstrated that the distribution of ACT1 and ACT3 mRNAs was very similar: both preferentially accumulated at high levels in mature pollen and at very low levels in the other major organs. The 5' flanking regions of both genes, including the promoter, leader exon and intron, and the first 19 condons, were fused to the beta-glucuronidase (GUS) reporter gene. The expression of these reporter fusions was examined in a large number of transgenic Arabidopsis plants. Histochemical assays demonstrated that both ACT1-GUS and ACT3-GUS constru...Continue Reading

References

May 1, 1992·The Plant Cell·J L CarpenterC D Silflow
Aug 1, 1990·Plant Molecular Biology·D McElroyR Wu
Apr 1, 1990·The Plant Cell·B G McLeanR B Meagher
May 15, 1987·Analytical Biochemistry·J LogemannL Willmitzer
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M A FrohmanG R Martin
Jan 27, 1989·Cell·E M Meyerowitz
Feb 1, 1989·The Journal of Cell Biology·M Kozak
Jun 1, 1988·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·W J Dickinson
Jun 1, 1987·The Journal of Cell Biology·B A Palevitz
Sep 1, 1987·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D R Cavener
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Oct 1, 1995·The Plant Journal : for Cell and Molecular Biology·E C McKinneyR B Meagher
Apr 1, 1994·Molecular and Cellular Biology·M M Tanzer, R B Meagher

❮ Previous
Next ❯

Citations

Jan 1, 2003·The Arabidopsis Book·Richard B Meagher, Marcus Fechheimer
Feb 16, 2005·Molecular Genetics and Genomics : MGG·Jean-Guy BerrinMichael Kazmaier
Jul 3, 1999·Trends in Genetics : TIG·R B MeagherA V Vitale
Dec 5, 1998·The Plant Journal : for Cell and Molecular Biology·E LebelE Ward
May 10, 2002·The Plant Journal : for Cell and Molecular Biology·Tokitaka OyamaKiyotaka Okada
Jan 22, 2003·The Plant Journal : for Cell and Molecular Biology·Laura U GillilandRichard B Meagher
Jan 26, 2002·Molecular Biology of the Cell·Muthugapatti K KandasamyRichard B Meagher
Oct 24, 2008·Phytopathology·C Vieira Dos SantosP Thalouarn
Mar 14, 2002·Plant Physiology·Elizabeth Cohen McKinneyRichard B Meagher
Oct 19, 2004·Plant Physiology·Megumi IwanoAkira Isogai
May 29, 2009·Plant Physiology·Megumi IwanoSeiji Takayama
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Peter W. Barlow, Frantisek Baluska
Feb 16, 2010·Biotechnology Advances·Priti N DesaiHarish Padh
Apr 26, 2003·The Plant Journal : for Cell and Molecular Biology·Barbara DespresMartine Devic
May 1, 2008·Plant Signaling & Behavior·Richard B MeagherElizabeth C McKinney
Feb 4, 2012·Plant Signaling & Behavior·Makoto T FujiwaraRyuuichi D Itoh
Jan 22, 2002·Metabolic Engineering·Philip A LessardAnthony J Sinskey
May 18, 2016·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Wolfgang GoettelYong-Qiang Charles An
Oct 13, 2006·Plant Molecular Biology·Lucia Cardenas PawloskiRichard Brian Meagher
Jun 23, 2015·The Plant Journal : for Cell and Molecular Biology·Ming Chang, Shanjin Huang
Jun 9, 2018·Plant Molecular Biology·Rozalynne SamiraTerri A Long
Sep 25, 2009·Molecular Biology Reports·Hui PengHao Ma
Jun 25, 2004·Plant & Cell Physiology·Anouck DietChristoph Ringli

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.