Conserved mechanism of Wnt signaling function in the specification of vulval precursor fates in C. elegans and C. briggsae

Developmental Biology
Ashwin SeetharamanBhagwati P Gupta

Abstract

The C. elegans hermaphrodite vulva serves as a paradigm for understanding how signaling pathways control organ formation. Previous studies have shown that Wnt signaling plays important roles in vulval development. To understand the function and evolution of Wnt signaling in Caenorhabditis nematodes we focused on C. briggsae, a species that is substantially divergent from C. elegans in terms of the evolutionary time scale yet shares almost identical morphology. We isolated mutants in C. briggsae that display multiple pseudo-vulvae resulting from ectopic VPC induction. We cloned one of these loci and found that it encodes an Axin homolog, Cbr-PRY-1. Our genetic studies revealed that Cbr-pry-1 functions upstream of the canonical Wnt pathway components Cbr-bar-1 (beta-catenin) and Cbr-pop-1(tcf/lef) as well as the Hox target Cbr-lin-39 (Dfd/Scr). We further characterized the pry-1 vulval phenotype in C. briggsae and C. elegans using 8 cell fate markers, cell ablation, and genetic interaction approaches. Our results show that ectopically induced VPCs in pry-1 mutants adopt 2° fates independently of the gonad-derived inductive and LIN-12/Notch-mediated lateral signaling pathways. We also found that Cbr-pry-1 mutants frequently show a...Continue Reading

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Citations

Nov 7, 2013·Molecular Biology and Evolution·Qing WeiRonald E Ellis
Dec 19, 2013·PLoS Genetics·Jordan D WardKeith R Yamamoto
Feb 14, 2012·Trends in Genetics : TIG·Marie-Anne Félix, Michalis Barkoulas
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Jan 1, 2013·G3 : Genes - Genomes - Genetics·Devika SharanyaBhagwati P Gupta
Jul 17, 2019·BMC Developmental Biology·Avijit MallickBhagwati P Gupta
Oct 18, 2019·Journal of Developmental Biology·Avijit MallickBhagwati P Gupta
Oct 2, 2019·Journal of Developmental Biology·Emily A Baker, Alison Woollard
Jan 22, 2020·Molecular Biology and Evolution·Helen M ChamberlinBhagwati P Gupta
Aug 9, 2012·Physical Biology·Marie-Anne Félix
Jul 25, 2018·G3 : Genes - Genomes - Genetics·Stéphanie GrimbertChristian Braendle
May 13, 2020·Biology Methods and Protocols·Elizabeth CulpBhagwati P Gupta

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