Conserved microRNA miR-8 in fat body regulates innate immune homeostasis in Drosophila

Developmental and Comparative Immunology
In Kyou Choi, Seogang Hyun

Abstract

Antimicrobial peptides (AMPs) constitute a major arm of the innate immune system across diverse organisms. In Drosophila, septic injury by microbial pathogens rapidly induces the production of the AMPs in fat body via well elucidated pathways such as Toll and IMD. However, several epithelial tissues were reported to locally express AMPs without septic injury via poorly characterized ways. Here, we report that microRNA miR-8 regulates the levels of AMPs basally expressed in Drosophila. The levels of AMPs such as Drosomycin and Diptericin are significantly increased in miR-8 null animals in non-pathogen stimulated conditions. Analysis of various larval tissues revealed that the increase of Drosomycin is fat body specific. Supporting this observation, re-introduction of miR-8 only in the fat body restored the altered AMP expression in miR-8 null flies. Although loss of miR-8 impedes PI3K in the fat body, inhibition of PI3K does not phenocopy the AMP expression of miR-8 null flies, indicating that miR-8 regulates AMP independently of PI3K. Together, our findings suggest a role of miR-8 in systemic immune homeostasis in generally non-pathogenic conditions in flies.

References

Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·B LemaitreJ A Hoffmann
May 30, 1998·The Journal of Biological Chemistry·E NicolasB Lemaitre
Mar 4, 2000·Insect Biochemistry and Molecular Biology·H Jiang, M R Kanost
Jul 23, 2003·Nature Immunology·Alexander N R WeberNicholas J Gay
Jun 16, 2004·Proceedings of the National Academy of Sciences of the United States of America·Xiaodi HuY Tony Ip
Mar 17, 2005·Frontiers in Bioscience : a Journal and Virtual Library·Mark D Marsden, R E K Fournier
May 16, 2006·Immunobiology·Lihui Wang, Petros Ligoxygakis
Oct 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Kate SengerMike Levine
Oct 20, 2006·Proceedings of the National Academy of Sciences of the United States of America·Myungjin KimJongkyeong Chung
Oct 31, 2006·Oncogene·S Minakhina, R Steward
Apr 18, 2007·Molecular and Cellular Biology·Takahiro TanjiY Tony Ip
May 18, 2007·Annual Review of Cell and Developmental Biology·Natascha Bushati, Stephen M Cohen
Oct 20, 2007·Nature Reviews. Immunology·Dominique FerrandonJules A Hoffmann
Oct 29, 2008·Genome Research·Robin C FriedmanDavid P Bartel
Jan 8, 2009·Science Signaling·Yashwanth Ashok
Apr 25, 2009·PLoS Genetics·Julie Hull-ThompsonHeinrich Jasper
Jun 7, 2011·Developmental and Comparative Immunology·Ane Fullaondo, So Young Lee

❮ Previous
Next ❯

Citations

Aug 13, 2013·Journal of Invertebrate Pathology·Diveena VijayendranBryony C Bonning
Apr 25, 2014·Lipids in Health and Disease·Odunayo Ibraheem AzeezJoseph Panashe Chamunorwa
Feb 13, 2015·Virusdisease·Claudia RückertRennos Fragkoudis
Nov 21, 2012·Insect Biochemistry and Molecular Biology·Keira Lucas, Alexander S Raikhel
Aug 8, 2015·Current Opinion in Insect Science·Keira J LucasAlexander S Raikhel
Dec 3, 2014·Developmental and Comparative Immunology·Nathan J DennisonGeorge Dimopoulos
Apr 4, 2014·Developmental and Comparative Immunology·Gang Jun Lee, Seogang Hyun
Oct 30, 2012·Insect Biochemistry and Molecular Biology·Sassan Asgari
Aug 26, 2014·Journal of Insect Physiology·Mazhar Hussain, Sassan Asgari
Jan 15, 2015·Scientific Reports·Roberta Peres da SilvaLysangela R Alves
Sep 10, 2013·Wiley Interdisciplinary Reviews. Developmental Biology·Kaushiki P MenonKai Zinn
Aug 24, 2018·International Journal of Molecular Sciences·Kyung-Jin Min, Marc Tatar
Nov 29, 2016·PLoS Pathogens·Xiao-Peng XiongRui Zhou
Jan 13, 2017·Applied Biochemistry and Biotechnology·Hongyan RenXinmin Zheng

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.