Antimicrobial peptides (AMPs) constitute a major arm of the innate immune system across diverse organisms. In Drosophila, septic injury by microbial pathogens rapidly induces the production of the AMPs in fat body via well elucidated pathways such as Toll and IMD. However, several epithelial tissues were reported to locally express AMPs without septic injury via poorly characterized ways. Here, we report that microRNA miR-8 regulates the levels of AMPs basally expressed in Drosophila. The levels of AMPs such as Drosomycin and Diptericin are significantly increased in miR-8 null animals in non-pathogen stimulated conditions. Analysis of various larval tissues revealed that the increase of Drosomycin is fat body specific. Supporting this observation, re-introduction of miR-8 only in the fat body restored the altered AMP expression in miR-8 null flies. Although loss of miR-8 impedes PI3K in the fat body, inhibition of PI3K does not phenocopy the AMP expression of miR-8 null flies, indicating that miR-8 regulates AMP independently of PI3K. Together, our findings suggest a role of miR-8 in systemic immune homeostasis in generally non-pathogenic conditions in flies.
The dorsoventral regulatory gene cassette spätzle/Toll/cactus controls the potent antifungal response in Drosophila adults
Drosophila host defense: differential induction of antimicrobial peptide genes after infection by various classes of microorganisms
A drosomycin-GFP reporter transgene reveals a local immune response in Drosophila that is not dependent on the Toll pathway
Regulation of imaginal disc cell size, cell number and organ size by Drosophila class I(A) phosphoinositide 3-kinase and its adaptor
The homeobox gene Caudal regulates constitutive local expression of antimicrobial peptide genes in Drosophila epithelia.
Lung V20 measurements: In regard to Seppenwoolde et al. (Int J Radiat Oncol Biol Phys 2003;55:724-735), and Tsujino et al. (Int J Radiat Oncol Biol Phys 2003;56:1208-1209)
Drosophila immunity: a large-scale in vivo RNAi screen identifies five serine proteases required for Toll activation
Boosting NF-kappaB-dependent basal immunity of Anopheles gambiae aborts development of Plasmodium berghei
Dual detection of fungal infections in Drosophila via recognition of glucans and sensing of virulence factors
The Drosophila systemic immune response: sensing and signalling during bacterial and fungal infections
Sequence relationships among C. elegans, D. melanogaster and human microRNAs highlight the extensive conservation of microRNAs in biology.
Regulation of aeroallergen immunity by the innate immune system: laboratory evidence for a new paradigm.
Re: "Metabolic Syndrome and Cognitive Performance Among Chinese ≥50 Years: A Cross-Sectional Study with 3988 Participants" by Chen et al. (Metab Syndr Relat Disord 2016;14:222-227)
The identification and characteristics of immune-related microRNAs in haemocytes of oyster Crassostrea gigas
Identification and profiling of Manduca sexta microRNAs and their possible roles in regulating specific transcripts in fat body, hemocytes, and midgut
MicroRNA-regulation of Anopheles gambiae immunity to Plasmodium falciparum infection and midgut microbiota
Identification of conserved and novel microRNAs in Manduca sexta and their possible roles in the expression regulation of immunity-related genes
Conserved microRNA miR-8 blocks activation of the Toll pathway by upregulating Serpin 27 transcripts
Identification and characterization of microRNAs in the screwworm flies Cochliomyia hominivorax and Cochliomyia macellaria (Diptera: Calliphoridae)
Identification and characterization of microRNAs expressed in the African malaria vector Anopheles funestus life stages using high throughput sequencing
De novo characterization of microRNAs in oriental fruit moth Grapholita molesta and selection of reference genes for normalization of microRNA expression
Lipopolysaccharide-Induced Differential Expression of miRNAs in Male and Female Rhipicephalus haemaphysaloides Ticks
Multiple functions of miR-8-3p in the development and metamorphosis of the red flour beetle, Tribolium castaneum
miR-958 inhibits Toll signaling and Drosomycin expression via direct targeting of Toll and Dif in Drosophila melanogaster
Transcriptome-Wide Identification of MicroRNAs and Analysis of Their Potential Roles in Development of Indian Meal Moth (Lepidoptera:Pyralidae)
Drosophila Myc restores immune homeostasis of Imd pathway via activating miR-277 to inhibit imd/Tab2
A Novel miRNA-hlo-miR-2-Serves as a Regulatory Factor That Controls Molting Events by Targeting CPR1 in Haemaphysalis longicornis Nymphs
An IMD-like pathway mediates both endosymbiont control and host immunity in the cereal weevil Sitophilus spp
The Drosophila miR-959-962 Cluster Members Repress Toll Signaling to Regulate Antibacterial Defense during Bacterial Infection.
Identification of wnt2 in the pearl mussel Hyriopsis cumingii and its role in innate immunity and gonadal development.
This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.