Sep 23, 2014

Conserved microsatellites in ants enable population genetic and colony pedigree studies across a wide range of species

PloS One
Ian A ButlerDaniel J C Kronauer

Abstract

Broadly applicable polymorphic genetic markers are essential tools for population genetics, and different types of markers have been developed for this purpose. Microsatellites have been employed as particularly polymorphic markers for over 20 years. However, PCR primers for microsatellite loci are often not useful outside the species for which they were designed. This implies that a new set of loci has to be identified and primers developed for every new study species. To overcome this constraint, we identified 45 conserved microsatellite loci based on the eight currently available ant genomes and designed primers for PCR amplification. Among these loci, we chose 24 for in-depth study in six species covering six different ant subfamilies. On average, 11.16 of these 24 loci were polymorphic and in Hardy-Weinberg equilibrium in any given species. The average number of alleles for these polymorphic loci within single populations of the different species was 4.59. This set of genetic markers will thus be useful for population genetic and colony pedigree studies across a wide range of ant species, supplementing the markers available for previously studied species and greatly facilitating the study of the many ant species lacking ge...Continue Reading

  • References49
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Mentioned in this Paper

Harpegnathos saltator
Short Tandem Repeat
Ectatomma ruidum
Tandem Repeat Sequences
Genome
Wasmannia auropunctata
Parvocaulis clavata
Dysequilibrium Syndrome
Genetics, Population
Ponerinae

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