Conserved motifs of MutL proteins

Mutation Research
Michał Banasik, Paweł Sachadyn

Abstract

The MutL protein is best known for its function in DNA mismatch repair (MMR). However, there is evidence to suggest that MutL is not only the linker connecting the functions of MutS and MutH in MMR, but that it also participates in other repair systems, such as Very Short Patch (VSP), Base Excision (BER) and Nucleotide Excision Repair (NER). This study set out to identify the most highly conserved amino acid sequence motifs in MutL proteins. We analyzed 208 MutL amino acid sequences of 199 representative prokaryotic species belonging to 28 classes of bacteria and archaea. The analysis revealed 16 conserved motifs situated in the ATPase and endonuclease domains, as well as within the disordered loop, and in the MutL regions interacting with the β clamp of DNA polymerase III. The conserved sequence motifs thus determined constitute a structural definition of MutL and they may be used in site-directed mutagenesis studies. We found conserved residues within the potential regions where binding with MutS occurs. However, the existing data does not provide clues as to the possible sites of MutL interactions with the proteins involved in other DNA repair systems such as NER, BER and VSP. We determined the 57 most highly conserved amino...Continue Reading

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Citations

Jan 5, 2016·DNA Repair·Peter FriedhoffJulia Gotthardt
Jan 28, 2017·Nature Communications·A Castañeda-GarcíaJ Blázquez
Apr 8, 2018·Biochemistry. Biokhimii︠a︡·M V MonakhovaE A Kubareva
Aug 7, 2020·International Journal of Molecular Sciences·Fabian CajaPavel Vodicka
Dec 2, 2020·Open Biology·Patricia Pérez-ArnaizThorsten Allers

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