Conserved mycobacterial lipoglycoproteins activate TLR2 but also require glycosylation for MHC class II-restricted T cell activation.

The Journal of Immunology : Official Journal of the American Association of Immunologists
Peter A SielingRobert L Modlin

Abstract

CD4(+) T cell clones derived from a leprosy lesion and patient blood were used to monitor the isolation and identification of an Ag associated with the self-limited form of the disease. Biochemical purification and genetic analysis identified the T cell Ag as a conserved mycobacterial lipoglycoprotein LprG. LprG-mediated activation of CD4(+) T cells required specific MHC class II restriction molecules and intracellular processing. Although LprG activated TLR2, this alone was not sufficient to stimulate or inhibit T cell activation. A striking finding was that the carbohydrate moieties of LprG were required for optimal T cell activation, because recombinant LprG produced in Escherichia coli, or recombinant LprG produced in Mycobacterium smegmatis and digested by alpha-mannosidase, did not activate T cells. This study demonstrates that the universe of bacterial T cell Ags includes lipoglycoproteins, which act as TLR2 ligands but also require glycosylation for MHC class II-restricted T cell activation in vivo.

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Citations

Jun 23, 2010·Molecular BioSystems·Clara EspitiaRaúl Mancilla
Jul 19, 2011·BMC Infectious Diseases·María V BiancoFabiana Bigi
May 29, 2016·Glycobiology·Lina SunFikri Y Avci
Apr 4, 2013·Proceedings of the National Academy of Sciences of the United States of America·Chia-Fang LiuMichel Rivière
May 24, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Laure ToniniMichel Rivière
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Sep 4, 2014·Microbiology and Molecular Biology Reviews : MMBR·Hanne L P Tytgat, Sarah Lebeer
Feb 23, 2021·Frontiers in Microbiology·Anna Allué-GuardiaJordi B Torrelles
Feb 7, 2009·Journal of Proteome Research·Margarita González-ZamoranoClara Espitia

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