Conserved nucleotides of 23 S rRNA located at the ribosomal peptidyltransferase center.

The Journal of Biological Chemistry
C M SpahnK H Nierhaus

Abstract

Two nucleotides of the 23 S rRNA gene were mutated; the nucleotides correspond to the first two positions of the universally conserved sequence PsiGG2582 at the peptidyltransferase ring of 23 S rRNA. The ribosomes containing the altered 23 S rRNA were analyzed. Previously, it was shown that ribosomal assembly was indistinguishable from that in wild-type cells, that the flow of the corresponding 50 S subunit into the polysome fraction was not restricted, but that the ribosomes were strongly impaired in poly(Phe) synthesis (C. M. T. Spahn, J. Remme, M. A. Schäfer, and K. H. Nierhaus (1996) J. Biol. Chem. 271, 32849-32856). Here we apply assay systems exclusively testing the puromycin reaction of ribosomes carrying plasmid-born rRNA, a dipeptide assay using the minimal P site donor pA(fMet) and a translocation system not depending on the puromycin reaction. The mutations in helix 90 exclusively abolish or severely impair the ribosome capability to catalyze AcPhe-puromycin formation. A possible explanation of these observations is that G2581 and Psi2580 (and possibly also G2582) are part of the binding site of C75 of peptidyl-tRNA in the P site. The results suggest that in this case, however, such an interaction would disobey canon...Continue Reading

References

Jan 5, 1991·Journal of Molecular Biology·B Stebbins-Boaz, S A Gerbi
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·D Moazed, H F Noller
Nov 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·R E MonroK A Marcker
Jul 1, 1993·Nucleic Acids Research·R R GutellM N Schnare
Dec 20, 1996·The Journal of Biological Chemistry·C M SpahnK H Nierhaus

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Citations

Sep 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·M Clelia GanozaHiroyuki Aoki
Apr 9, 1999·Biological Chemistry·S Dorner, A Barta
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·D R BenjaminC M Dobson
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·M BocchettaA S Mankin
Jan 5, 2000·Molecular Cell·D F Kim, R Green
Dec 20, 1996·The Journal of Biological Chemistry·C M SpahnK H Nierhaus
May 13, 1999·The Journal of Biological Chemistry·A M KrichevskyH Rosen
Nov 16, 2002·The Journal of Biological Chemistry·John DresiosDennis Synetos
Aug 15, 1998·Biological Chemistry·C M Spahn, K H Nierhaus
Apr 7, 2018·Microbiology Spectrum·Zhangqi ShenJianzhong Shen
Nov 11, 2021·Microbiology and Molecular Biology Reviews : MMBR·Madhan R TirumalaiGeorge E Fox

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