Conserved peptide vaccine candidates containing multiple Ebola nucleoprotein epitopes display interactions with diverse HLA molecules

Medical Microbiology and Immunology
Sahil Jain, Manoj Baranwal

Abstract

Immunoinformatics has come by leaps and bounds to finding potent vaccine candidates against various pathogens. In the current study, a combination of different T (CD4+ and CD8+) and B cell epitope prediction tools was applied to find peptides containing multiple epitopes against Ebola nucleoprotein (NP) and the presentation of peptides to human leukocyte antigen (HLA) molecules was analyzed by prediction, docking and population coverage tools. Further, potential peptides were analyzed by ELISA for peptide induced IFN-γ secretion in peripheral blood mononuclear cells isolated from healthy volunteers. Six peptides were obtained after merging the overlapping multiple HLA I (CD8+) and II (CD4+) restricted T cell epitopes as well as B cell epitopes and eliminating the peptides liable to generate autoimmune and allergic response. All peptides displayed 100% conservancy in Zaire ebolavirus. In other Ebola virus species (Sudan, Bundibugyo and Taï forest) and Filoviridae members (Lloviuvirus and Margburgvirus), some peptides were found to be conserved with minor variations. Prediction tools confirmed the ability of predicted peptides to bind with diverse HLA (HLA-A, HLA-B, HLA-DP, HLA-DQ and HLA-DR) alleles. CABS-dock results displayed ...Continue Reading

References

Jan 1, 1994·Canadian Journal of Physiology and Pharmacology·G S MorrisW M Sherman
Dec 1, 1996·Journal of Autoimmunity·A D Cohen, Y Shoenfeld
Dec 22, 1999·Immunogenetics·H RammenseeS Stevanović
Feb 7, 2001·Pediatrics·A PatjaH Peltola
Dec 26, 2001·Bioinformatics·H Singh, G P Raghava
Feb 13, 2004·Bioinformatics·Manoj Bhasin, G P S Raghava
Feb 20, 2004·Virology·Graham SimmonsHao Shen
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Mar 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Manisha GuptaPierre E Rollin
Mar 31, 2006·Journal of Virology·Shinji WatanabeYoshihiro Kawaoka
Jun 17, 2006·Science·Aurélie A V AlbertiniRob W H Ruigrok
Jul 18, 2006·Nucleic Acids Research·Sudipto Saha, G P S Raghava
Nov 2, 2007·BMC Bioinformatics·Mette V LarsenMorten Nielsen
Jan 25, 2008·Clinical and Vaccine Immunology : CVI·Dana L SwensonWilliam D Pratt
Feb 19, 2010·The Journal of General Virology·Takeshi NodaYoshihiro Kawaoka
Feb 10, 2012·Journal of Clinical Immunology·Jacob AtsmonTamar Ben-Yedidia
Aug 12, 2014·Nature Structural & Molecular Biology·Filip YabukarskiMarc Jamin
Nov 22, 2014·Nucleic Acids Research·James RobinsonSteven G E Marsh
Nov 27, 2014·The New England Journal of Medicine·Julie E LedgerwoodUNKNOWN VRC 207 Study Team
Jan 30, 2015·The New England Journal of Medicine·Katie EwerAdrian V S Hill
Apr 2, 2015·Proceedings of the National Academy of Sciences of the United States of America·Maher AlayyoubiRobert A Lamb
Jun 30, 2015·Cell Reports·Robert N KirchdoerferErica Ollmann Saphire
Jul 8, 2015·Applied Microbiology and Biotechnology·Prachiti AfleySubodh Kumar
Jul 15, 2015·Methods : a Companion to Methods in Enzymology·Maciej BlaszczykSebastian Kmiecik

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Methods Mentioned

BETA
ELISA

Software Mentioned

Discovery
Motif Alignment & Search Tool
SYFPEITHI
Dock
AVANA
IEDB
CABS
MUSCLE
Algpred
Discovery studio Visualizer

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