PMID: 9423925Jan 10, 1998Paper

Conserved phosphorylation of the intracellular domains of GABA(A) receptor beta2 and beta3 subunits by cAMP-dependent protein kinase, cGMP-dependent protein kinase protein kinase C and Ca2+/calmodulin type II-dependent protein kinase

Neuropharmacology
B J McDonald, S J Moss

Abstract

All mammalian GABA(A) receptor beta subunits contain a conserved consensus site for phosphorylation by a number of serine/threonine protein kinases. This site corresponds to Serine 410 of the beta2 subunit and Serine 409 of the beta3 subunit, each of which lies within the conserved sequence R-R-R-X-S-L-Q-K, where X = A (beta1, beta2 and beta4) or S (beta3). We have analysed the phosphorylation of the beta2 and beta3 subunits of the murine GABA(A) receptor by expressing the large intracellular domains of these subunits as soluble fusion proteins in E. coli. The intracellular domain of the beta2 subunit was phosphorylated to high stoichiometry by both cAMP- and cGMP-dependent protein kinases, protein kinase C and Ca2+/calmodulin type II-dependent protein kinase in vitro. Site-directed mutagenesis identified Serine 410 as the single site within the beta2 subunit phosphorylated by these four protein kinases. Using similar methodologies, Serine 409 of the beta3 subunit was shown to be a substrate for phosphorylation by these protein kinases. Serine 408 was also seen to be phosphorylated by protein kinase C and Serine 383 was phosphorylated by Ca2+/calmodulin type II-dependent protein kinase. Since beta subunits are believed to be es...Continue Reading

References

Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P WhitingL L Iversen
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M D BrowningR W Olsen
Jul 31, 1990·European Journal of Pharmacology·S J MossE A Barnard
Jan 1, 1987·Methods in Enzymology·T A KunkelR A Zakour
Jul 1, 1995·Brain Research. Molecular Brain Research·C F ValenzuelaR A Harris
Jan 1, 1994·Annual Review of Neuroscience·R L Macdonald, R W Olsen
Sep 28, 1995·Nature·S J MossT G Smart
Aug 15, 1995·The Biochemical Journal·F A Stephenson
Apr 1, 1993·Trends in Neurosciences·L A RaymondR L Huganir
Mar 14, 1995·Biochimica Et Biophysica Acta·G L KamatchiD R Burt
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·C N ConnollyS J Moss

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Citations

Mar 20, 2004·Pharmacology & Therapeutics·Sandeep KumarA Leslie Morrow
Sep 19, 2003·International Journal for Parasitology·Darran M YatesAdrian J Wolstenholme
Aug 15, 2002·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Anna Sedelnikova, David S Weiss
Mar 2, 2002·Journal of Diabetes and Its Complications·Naoaki SaitoYasutomi Nishizuka
Sep 21, 2010·Quarterly Reviews of Biophysics·Andrew J ThompsonSarah C R Lummis
Nov 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Kurt C MarsdenReed C Carroll
Aug 19, 2010·Pharmacological Reviews·Sharron H FrancisDavid Sibley
Dec 22, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Xiao Hu ZhangHsiao Chang Chan
Sep 30, 2005·Proceedings of the National Academy of Sciences of the United States of America·Josef T KittlerStephen J Moss
Feb 26, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Darren GoffinJasmina N Jovanovic
Nov 6, 2004·The Journal of Physiology·Istvan Mody
Apr 1, 2009·The Journal of Physiology·Catriona M HoustonTrevor G Smart
Oct 28, 2005·Cardiovascular Drug Reviews·Lindon H YoungMargaret T Weis
Mar 3, 2004·Annals of the New York Academy of Sciences·Arjen B Brussaard, Jan-Jurjen Koksma
Nov 15, 2006·The European Journal of Neuroscience·C M Houston, T G Smart
Oct 3, 2006·Journal of Neurobiology·Cathy L Bell-HornerMeharvan Singh
Jul 13, 2004·Pharmacology & Therapeutics·Bernhard Lüscher, Cheryl A Keller
Aug 25, 2015·The European Journal of Neuroscience·Nico AnglikerMarkus A Rüegg
Jun 24, 2003·Neuron·Qinghua WangYu Tian Wang
May 15, 2012·Bioorganic & Medicinal Chemistry Letters·Gary E KeckPeter M Blumberg
Jan 10, 2006·Cell Metabolism·Elaine XuQinghua Wang
Nov 12, 2014·Frontiers in Cellular Neuroscience·Carmen E Flores, Pablo Méndez
Jan 25, 2014·Molecular Brain·Jun Liu, Yu Tian Wang
Nov 19, 2013·PloS One·William M ConnellyVincenzo Crunelli

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