Conserved requirement for DEAD-box RNA helicase Gemin3 in Drosophila oogenesis.

BMC Research Notes
Ruben J Cauchi

Abstract

DEAD-box RNA helicase Gemin3 is an essential protein since its deficiency is lethal in both vertebrates and invertebrates. In addition to playing a role in transcriptional regulation and RNA silencing, as a core member of the SMN (survival of motor neurons) complex, Gemin3 is required for the biogenesis of spliceosomal snRNPs (small nuclear ribonucleoproteins), and axonal mRNA metabolism. Studies in the mouse and C. elegans revealed that loss of Gemin3 function has a negative impact on ovarian physiology and development. This work reports on the generation and characterisation of gemin3 mutant germline clones in Drosophila adult females. Gemin3 was found to be required for the completion of oogenesis and its loss led to egg polarity defects, oocyte mislocalisation, and abnormal chromosome morphology. Canonical Cajal bodies were absent in the majority of gemin3 mutant egg chambers and histone locus bodies displayed an atypical morphology. snRNP distribution was perturbed so that on gemin3 loss, snRNP cytoplasmic aggregates (U bodies) were only visible in wild type. These findings establish a conserved requirement for Gemin3 in Drosophila oogenesis. Furthermore, in view of the similarity to the phenotypes described previously in ...Continue Reading

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Citations

Feb 14, 2014·Wiley Interdisciplinary Reviews. RNA·Jennifer A Schisa
Mar 22, 2014·CNS Neuroscience & Therapeutics·Ruben J Cauchi
Nov 2, 2014·Frontiers in Neuroscience·Rebecca Borg, Ruben J Cauchi
Mar 11, 2018·Biochemical Society Transactions·Frank Curmi, Ruben J Cauchi
Jun 20, 2020·Molecular & Cellular Proteomics : MCP·Prashali BansalFulvia Bono
Jan 17, 2021·Molecular & Cellular Proteomics : MCP·Prashali BansalFulvia Bono

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Cajal Bodies & Gems

Cajal bodies or coiled bodies are dense foci of coilin protein. Gemini of Cajal bodies, or gems, are microscopically similar to Cajal bodies. It is believed that Cajal bodies play important roles in RNA processing while gems assist the Cajal bodies. Find the latest research on Cajal bodies and gems here.