Conserved residues Asp16 and Pro24 of TnaC-tRNAPro participate in tryptophan induction of Tna operon expression.

Journal of Bacteriology
Luis Cruz-Vera, Charles Yanofsky

Abstract

In Escherichia coli, interactions between the nascent TnaC-tRNA(Pro) peptidyl-tRNA and the translating ribosome create a tryptophan binding site in the ribosome where bound tryptophan inhibits TnaC-tRNA(Pro) cleavage. This inhibition delays ribosome release, thereby inhibiting Rho factor binding and action, resulting in increased tna operon transcription. Replacing Trp12 of TnaC with any other amino acid residue was previously shown to prevent tryptophan binding and induction of tna operon expression. Genome-wide comparisons of TnaC amino acid sequences identify Asp16 and Pro24, as well as Trp12, as highly conserved TnaC residues. Replacing these residues with other residues was previously shown to influence tryptophan induction of tna operon expression. In this study, in vitro analyses were performed to examine the potential roles of Asp16 and Pro24 in tna operon induction. Replacing Asp16 or Pro24 of TnaC of E. coli with other amino acids established that these residues are essential for free tryptophan binding and inhibition of TnaC-tRNA(Pro) cleavage at the peptidyl transferase center. Asp16 and Pro24 are in fact located in spatial positions corresponding to critical residues of AAP, another ribosome regulatory peptide. Spa...Continue Reading

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Citations

Oct 5, 2010·The Journal of Biological Chemistry·Christina C SpevakMatthew S Sachs
May 20, 2008·Journal of Bacteriology·Valley Stewart
Aug 4, 2009·Journal of Bacteriology·Bassam AbomoelakAdel M Talaat
Sep 22, 2009·Journal of Bacteriology·Luis R Cruz-VeraCharles Yanofsky
Jun 12, 2009·Annual Review of Microbiology·Heather M HoodMatthew S Sachs
Mar 16, 2011·Journal of Visualized Experiments : JoVE·Nitin ShiroleLuis Cruz-Vera
Jun 12, 2013·Annual Review of Biochemistry·Koreaki Ito, Shinobu Chiba
Feb 10, 2016·Current Opinion in Structural Biology·Daniel N WilsonRoland Beckmann
Sep 24, 2015·Proceedings of the National Academy of Sciences of the United States of America·Eiji IshiiHiroyuki Mori
Apr 29, 2015·Proceedings of the National Academy of Sciences of the United States of America·Michael T EnglanderVirginia W Cornish
Feb 24, 2011·Current Opinion in Microbiology·Luis Rogelio Cruz-VeraCharles Yanofsky
Sep 26, 2009·The EMBO Journal·Shinobu ChibaKit Pogliano
Jan 28, 2009·Molecular Microbiology·Haripriya RamuNora Vazquez-Laslop
May 4, 2011·Biotechnology Journal·Sujata Jha, Anton A Komar
Sep 19, 2009·Journal of Molecular Biology·Brian D Janssen, Christopher S Hayes
Oct 15, 2014·Cell Reports·Lukas BischoffRoland Beckmann
Feb 5, 2011·Molecular Cell·Daniel N Wilson
May 14, 2010·Structure·Leonardo G TrabucoKlaus Schulten
Jun 11, 2010·Chemistry & Biology·Agata L StarostaDaniel N Wilson
Feb 2, 2010·Biochemical and Biophysical Research Communications·Koreaki ItoKit Pogliano
Jul 5, 2019·Microbiology and Molecular Biology Reviews : MMBR·Charles L Turnbough
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Nov 28, 2015·Current Genetics·Yongxin JinWeihui Wu
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Sep 2, 2020·Annual Review of Genetics·Thomas E DeverMatthew S Sachs
Sep 11, 2021·Nature Communications·Anne-Xander van der StelC Axel Innis

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