Conserved sequence elements in K promoters from mice and humans: implications for transcriptional regulation and repertoire expression

Immunogenetics
M BemarkT Leanderson

Abstract

Promoter region sequences of human and mouse Igk-V genes were aligned and found to be conserved for about 200-300 base pairs (bp) within subgroups/families. No promoter similarity was found between IGKV promoters from different human subgroups. Related mouse Igk-V gene families were conserved in the promoter region but no similarity was evident when promoters from unrelated Igk-V gene families were compared. Most of the human IGKV promoter subgroups were shown to have mouse counterparts with a similarity region that extended about 150 bp upstream of the translational start codon. All promoters contained an octamer sequence element. The consensus octamer/decamer sequence was favored but only seven residues within the octamer element were strictly conserved. Furthermore, there was also sequence conservation immediately 3' of the octamer where either an A or a G residue was conserved. In addition, other DNA elements were also conserved both within the Igk-V subgroups/families and between mouse and human promoters from related subgroups/families. In several of the subgroups/families an E box of the E2A type was conserved 5' of the octamer and a CCCT element was conserved within the IGKV subgroup II and its related mouse Igk-V famil...Continue Reading

Citations

Sep 21, 2004·Immunogenetics·Katherine M Brekke, William T Garrard
Oct 16, 2002·Developmental and Comparative Immunology·Sirje TimmuskLars Pilström
Sep 26, 2006·Nature Reviews. Immunology·David Nemazee
Feb 15, 2001·The Biochemical Journal·A AranburuT Leanderson
Jul 10, 2004·Immunological Reviews·Ann J FeeneyCelia R Espinoza
Apr 3, 1999·The Journal of Biological Chemistry·M BemarkT Leanderson
Aug 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Peter GoebelAnn J Feeney
May 1, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shuji SakamotoYasutoshi Agata
Jun 28, 2021·Genes and Immunity·Ivana MikocziovaLudvig M Sollid

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