Conserved serine-rich sequences in xylanase and cellulase from Pseudomonas fluorescens subspecies cellulosa: internal signal sequence and unusual protein processing

Molecular Microbiology
J HallH J Gilbert

Abstract

The complete nucleotide sequence of the xynA gene coding for a xylanase (XYLA) expressed by Pseudomonas fluorescens subspecies cellulosa, has been determined. The structural gene consists of an open reading frame of 1833 bp followed by a TAA stop codon. Confirmation of the nucleotide sequence was obtained by comparing the predicted amino acid sequence with that derived by N-terminal analysis of purified forms of the xylanase. The signal peptide present at the N terminus of mature XYLA closely resembles signal peptides of other secreted proteins. Truncated forms of the xylanase gene, in which the sequence encoding the N-terminal signal peptide had been deleted, still expressed coli. XYLA contains domains which are homologous to an endoglucanase expressed by the same organism. These structures include serine-rich sequences. Bal31 deletions of xynA revealed the extent to which these conserved sequences, in XYLA, were essential for xylanase activity. Downstream of the TAA stop codon is a G + C-rich region of dyad symmetry (delta G = 24 kcal) characteristic of E. coli Rho-independent transcription terminators.

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Citations

May 1, 1992·Molecular & General Genetics : MGG·P E Vercoe, K Gregg
Jul 28, 2010·The Journal of Biological Chemistry·Cedric MontanierHarry J Gilbert
Mar 1, 1992·The Journal of Applied Bacteriology·G P HazlewoodH J Gilbert
Mar 28, 1991·European Journal of Biochemistry·A R Hawkins, M Smith
Dec 15, 1991·European Journal of Biochemistry·N R GilkesR C Miller
Aug 6, 1998·Bioscience, Biotechnology, and Biochemistry·K InagakiH Tanaka
Apr 13, 2004·Protein Science : a Publication of the Protein Society·Michael B HowardRonald M Weiner
May 26, 2010·Journal of Applied Microbiology·K Kylä-NikkiläP E J Saris
Mar 24, 2016·Phytopathology·Bode OlukoluPeter John Balint-Kurti
Jun 6, 2016·World Journal of Microbiology & Biotechnology·Jeffrey G Gardner
Oct 15, 1991·FEMS Microbiology Letters·J H ClarkeG P Hazlewood
Sep 1, 1990·Molecular & General Genetics : MGG·D M PooleH J Gilbert
Nov 21, 1998·The Journal of Biological Chemistry·S J CharnockH J Gilbert
Jul 11, 1997·The Journal of Biological Chemistry·T D SpurwayH J Gilbert
Jan 1, 1993·FEMS Microbiology Reviews·J A Thomson

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