Conserved structural elements in the V3 crown of HIV-1 gp120.

Nature Structural & Molecular Biology
Xunqing JiangXiang-Peng Kong

Abstract

Binding of the third variable region (V3) of the HIV-1 envelope glycoprotein gp120 to the cell-surface coreceptors CCR5 or CXCR4 during viral entry suggests that there are conserved structural elements in this sequence-variable region. These conserved elements could serve as epitopes to be targeted by a vaccine against HIV-1. Here we perform a systematic structural analysis of representative human anti-V3 monoclonal antibodies in complex with V3 peptides, revealing that the crown of V3 has four conserved structural elements: an arch, a band, a hydrophobic core and the peptide backbone. These are either unaffected by or are subject to minimal sequence variation. As these regions are targeted by cross-clade neutralizing human antibodies, they provide a blueprint for the design of vaccine immunogens that could elicit broadly cross-reactive protective antibodies.

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Citations

Sep 6, 2011·Journal of Chemical Information and Modeling·Alexander M AndrianovAlexander V Tuzikov
Feb 24, 2011·Journal of the American Chemical Society·Joan G SchellingerJacquelyn Gervay-Hague
Jun 26, 2010·Nature Reviews. Immunology·Susan Zolla-Pazner, Timothy Cardozo
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Jul 22, 2014·Vaccine·Timothy CardozoChavdar Krachmarov
Nov 21, 2013·Journal of Biomolecular Structure & Dynamics·Alexander M AndrianovAlexander V Tuzikov

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