Conserved white-rot enzymatic mechanism for wood decay in the Basidiomycota genus Pycnoporus.

DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes
Shingo MiyauchiMarie-Noëlle Rosso

Abstract

White-rot (WR) fungi are pivotal decomposers of dead organic matter in forest ecosystems and typically use a large array of hydrolytic and oxidative enzymes to deconstruct lignocellulose. However, the extent of lignin and cellulose degradation may vary between species and wood type. Here, we combined comparative genomics, transcriptomics and secretome proteomics to identify conserved enzymatic signatures at the onset of wood-decaying activity within the Basidiomycota genus Pycnoporus. We observed a strong conservation in the genome structures and the repertoires of protein-coding genes across the four Pycnoporus species described to date, despite the species having distinct geographic distributions. We further analysed the early response of P. cinnabarinus, P. coccineus and P. sanguineus to diverse (ligno)-cellulosic substrates. We identified a conserved set of enzymes mobilized by the three species for breaking down cellulose, hemicellulose and pectin. The co-occurrence in the exo-proteomes of H2O2-producing enzymes with H2O2-consuming enzymes was a common feature of the three species, although each enzymatic partner displayed independent transcriptional regulation. Finally, cellobiose dehydrogenase-coding genes were systemati...Continue Reading

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Citations

Nov 20, 2020·Molecular Biology and Evolution·Francisco J Ruiz-DueñasAngel T Martínez
Feb 5, 2021·Environmental Microbiology·Hayat HageMarie-Noëlle Rosso
Aug 27, 2021·Journal of Fungi·Mariane DaouCraig B Faulds

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Datasets Mentioned

BETA
VICQ00000000
GSE82486

Methods Mentioned

BETA
glycosylation
RNASeq
RNA-seq

Software Mentioned

ComplexHeatmap ’
DESeq2
R
R package kohonen
MAFFT
RAxML
FastOrtho
Blastx
R scripts
TopHat

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