Considerations for the use of virally delivered genetic tools for in-vivo circuit analysis and behavior in mutant mice: a practical guide to optogenetics

Behavioural Pharmacology
Dóra ZelenaDiána Balázsfi

Abstract

Optogenetics was the method of the year in 2010 according to Nature Neuroscience. Since then, this method has become widespread, the use of virally delivered genetic tools has extended to other fields such as pharmacogenetics, and optogenetic techniques have become frequently applied in genetically manipulated animals for in-vivo circuit analysis and behavioral studies. However, several issues should be taken into consideration when planning such experiments. We aimed to summarize the critical points concerning optogenetic manipulation of a specific brain area in mutant mice. First, the appropriate vector should be chosen to allow optimal optogenetic manipulation. Adeno-associated viral vectors are the most common carriers with different available serotypes. Light-sensitive channels are available in many forms, and the expression of the delivered genetic material can be influenced in many ways. Second, selecting the adequate stimulation protocol is also essential. The pattern, intensity, and timing could be determinative parameters. Third, the mutant strain might have a phenotype that influences the observed behavior. In conclusion, detailed preliminary experiments and numerous control groups are required to choose the best vec...Continue Reading

References

Nov 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Georg NagelErnst Bamberg
Dec 11, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Junichi IdenoKeiya Ozawa
Dec 11, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Haiyan FuDouglas M McCarty
May 10, 2005·Virology Journal·Manuel A F V Gonçalves
Apr 5, 2007·Nature Protocols·Joshua C GriegerR Jude Samulski
Jun 6, 2008·Journal of Virology·Magalie Penaud-BudlooRichard O Snyder
Oct 16, 2008·Clinical Microbiology Reviews·Shyam Daya, Kenneth I Berns
Oct 28, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jean-Marc GuettierJürgen Wess
Feb 12, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Bénédicte AmilhonSalah El Mestikawy
Nov 30, 2010·Journal of Visualized Experiments : JoVE·Rebecca L Lowery, Ania K Majewska
Feb 1, 2011·Nature Neuroscience·Ilka DiesterKrishna V Shenoy
Feb 18, 2011·IEEE Transactions on Bio-medical Engineering·Nir GrossmanPatrick Degenaar
May 26, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Hongwei ZhangGuangping Gao
Jul 13, 2011·Neuron·Ofer YizharKarl Deisseroth
Aug 7, 2012·Frontiers in Neural Circuits·Arpiar SaundersBernardo L Sabatini
Oct 13, 2012·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Ellen C Jensen
Oct 16, 2012·Brain Research·Martilias S Farrell, Bryan L Roth
Nov 29, 2012·Frontiers in Behavioral Neuroscience·Cornelia Schöne, Denis Burdakov
Jul 13, 2013·Frontiers in Cellular Neuroscience·Nicolas MerienneGilles Bonvento
Jan 31, 2014·Frontiers in Immunology·Karen Nieto, Anna Salvetti
Oct 7, 2014·Frontiers in Molecular Neuroscience·Giridhar MurlidharanAravind Asokan
Oct 9, 2014·Annual Review of Pharmacology and Toxicology·Daniel J Urban, Bryan L Roth
Nov 28, 2014·Current Protocols in Neuroscience·Bryan B GoreLarry S Zweifel
Feb 26, 2015·Frontiers in Integrative Neuroscience·Mark A RossiHenry H Yin
Jul 15, 2015·Cell Reports·Joseph M StujenskeJoshua A Gordon
Jul 21, 2015·Frontiers in Neuroanatomy·Jonathan J NassiKevin T Beier
Jul 21, 2015·Frontiers in Behavioral Neuroscience·Heinrich S GompfCaroline E Bass
Aug 1, 2015·Molecular Therapy : the Journal of the American Society of Gene Therapy·Tomomi KiyotaHoward E Gendelman
Aug 5, 2015·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·David OwaldScott Waddell

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Citations

Jul 7, 2018·Hippocampus·Charan Ranganath
Jul 6, 2019·FEMS Microbiology Ecology·Johanna GutlebenDetmer Sipkema

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