Considerations related to the use of short neuropeptide promoters in viral vectors targeting hypothalamic neurons

Scientific Reports
N Kakava-GeorgiadouR A H Adan

Abstract

Targeting specific neuronal cell types is a major challenge for unraveling their function and utilizing specific cells for gene therapy strategies. Viral vector tools are widely used to target specific cells or circuits for these purposes. Here, we use viral vectors with short promoters of neuropeptide genes to target distinct neuronal populations in the hypothalamus of rats and mice. We show that lowering the amount of genomic copies is effective in increasing specificity of a melanin-concentrating hormone promoter. However, since too low titers reduce transduction efficacy, there is an optimal titer for achieving high specificity and sufficient efficacy. Other previously identified neuropeptide promoters as those for oxytocin and orexin require further sequence optimization to increase target specificity. We conclude that promoter-driven viral vectors should be used with caution in order to target cells specifically.

References

Oct 31, 2000·Neuron·T C ThannickalJ M Siegel
Jun 6, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Dirk GrimmJuergen A Kleinschmidt
Dec 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Tanjew DittgenPavel Osten
Jan 16, 2010·Human Gene Therapy·Marijke W A de BackerRoger A H Adan
Jan 7, 2011·Nature Neuroscience·Yexica AponteScott M Sternson
Mar 3, 2011·The Journal of Clinical Investigation·Michael J KrashesBradford B Lowell
Sep 1, 2011·Neuroreport·Su-Ping PengMelitta Schachner
Jul 18, 2012·Nature·Deniz AtasoyScott M Sternson
Jun 21, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Roda Rani KonadhodePriyattam J Shiromani
Dec 19, 2013·Frontiers in Neural Circuits·Yuki C SaitoTakeshi Sakurai
Jan 23, 2015·Science Translational Medicine·Olga PeñagarikanoDaniel H Geschwind
Oct 13, 2016·Annual Review of Genetics·Lucas SjulsonGero Miesenböck

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Methods Mentioned

BETA
transgenic
PCR

Software Mentioned

ImageJ
GraphPad Prism

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