Consistent and reproducible long-term in vitro growth of health and disease-associated oral subgingival biofilms

BMC Microbiology
Irina M Velsko, L M Shaddox

Abstract

Several in vitro oral biofilm growth systems can reliably construct oral microbiome communities in culture, yet their stability and reproducibility through time has not been well characterized. Long-term in vitro growth of natural biofilms would enable use of these biofilms in both in vitro and in vivo studies that require complex microbial communities with minimal variation over a period of time. Understanding biofilm community dynamics in continuous culture, and whether they maintain distinct signatures of health and disease, is necessary to determine the reliability and applicability of such models to broader studies. To this end, we performed next-generation sequencing on biofilms grown from healthy and disease-site subgingival plaque for 80 days to assess stability and reliability of continuous oral biofilm growth. Biofilms were grown from subgingival plaque collected from periodontitis-affected sites and healthy individuals for ten eight-day long generations, using hydroxyapatite disks. The bacterial community in each generation was determined using Human Oral Microbe Identification by Next-Generation Sequencing (HOMINGS) technology, and analyzed in QIIME. Profiles were steady through the ten generations, as determined by...Continue Reading

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Citations

Jan 31, 2020·Journal of Dental Research·D BaraniyaN N Al-Hebshi
Jan 2, 2019·Journal of Oral Microbiology·Fabian CieplikRob A M Exterkate
Nov 12, 2020·Molecular Oral Microbiology·Eleanor I LamontJeffrey S McLean
Nov 8, 2020·Applied and Environmental Microbiology·Yaling JiangDong Mei Deng
Feb 11, 2021·Klinicheskaia laboratornaia diagnostika·V M ChervinetsA Yu Mironov
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Mar 11, 2021·Periodontology 2000·Nicholas S JakubovicsFabian Cieplik

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Clinical Trials Mentioned

NCT01330719

Software Mentioned

Statistical Analysis of Metagenomic Profiles ( STAMP )
ProbeSeq
uclust
HOMINGS
adonis
QIIME
vegan R package
STAMP

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