Consolidated bioprocessing of starchy substrates into ethanol by industrial Saccharomyces cerevisiae strains secreting fungal amylases

Biotechnology and Bioengineering
Lorenzo FavaroSergio Casella

Abstract

The development of a yeast strain that converts raw starch to ethanol in one step (called Consolidated Bioprocessing, CBP) could significantly reduce the commercial costs of starch-based bioethanol. An efficient amylolytic Saccharomyces cerevisiae strain suitable for industrial bioethanol production was developed in this study. Codon-optimized variants of the Thermomyces lanuginosus glucoamylase (TLG1) and Saccharomycopsis fibuligera α-amylase (SFA1) genes were δ-integrated into two S. cerevisiae yeast with promising industrial traits, i.e., strains M2n and MEL2. The recombinant M2n[TLG1-SFA1] and MEL2[TLG1-SFA1] yeast displayed high enzyme activities on soluble and raw starch (up to 8118 and 4461 nkat/g dry cell weight, respectively) and produced about 64 g/L ethanol from 200 g/L raw corn starch in a bioreactor, corresponding to 55% of the theoretical maximum ethanol yield (g of ethanol/g of available glucose equivalent). Their starch-to-ethanol conversion efficiencies were even higher on natural sorghum and triticale substrates (62 and 73% of the theoretical yield, respectively). This is the first report of direct ethanol production from natural starchy substrates (without any pre-treatment or commercial enzyme addition) usin...Continue Reading

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Citations

Jul 30, 2016·Applied Microbiology and Biotechnology·Steffi A DavisonWillem Heber van Zyl
Dec 12, 2018·FEMS Yeast Research·Rosemary A CripwellWillem H van Zyl
Jan 25, 2019·Applied Microbiology and Biotechnology·Monika BorkowskaEwelina Celińska
Mar 16, 2017·World Journal of Microbiology & Biotechnology·Pascal HumbertAnant V Patel
Dec 22, 2019·Biotechnology Advances·Xiujuan QianKatrin Ochsenreither
Aug 1, 2020·Journal of Environmental Management·Surbhi SharmaTejraj M Aminabhavi

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