PMID: 9551082Apr 29, 1998Paper

Constitutive and interferon-gamma-induced expression of the human proteasome subunit multicatalytic endopeptidase complex-like 1

Biochimica Et Biophysica Acta
G S FossH Prydz

Abstract

Proteasomes generate peptides from intracellular endogenous and viral proteins for presentation by MHC class I molecules. During viral infection, interferon-gamma (IFN-gamma) acts as a cytokine altering the catalytic specificity of proteasomes by inducing the synthesis of the three proteasome subunits, low molecular weight protein (LMP) 2, LMP7 and multicatalytic endopeptidase complex-like 1 (MECL1). LMP2 and LMP7 have been shown to favour the presentation of certain antigenic peptides. These subunits are constitutively expressed in cell lines related to the immune system and IFN-gamma-inducible in other cell lines. Less is known about MECL1. To reveal the extent of constitutive and IFN-gamma-induced expression of MECL1, we studied MECL1 in different cell lines by Northern and Western blotting. The two B cell lines IM9 and Reh showed high constitutive expression of MECL1, only slightly induced by IFN-gamma stimulation. The B cell line Daudi and the monocyte cell line THP-1 expressed MECL1 constitutively at an intermediate level. The MECL1 protein level in the THP-1 cells increased markedly in response to IFN-gamma. In cells unrelated to the immune system, a very low constitutive expression of MECL1 was detected, highly inducibl...Continue Reading

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Citations

Jul 18, 2006·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Shu-Hong ZhaoChristopher K Tuggle
Aug 28, 2001·Free Radical Biology & Medicine·Q Ding, J N Keller
May 3, 2002·European Journal of Biochemistry·Steven L ParkerMagnus M Berglund
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Nov 13, 2003·Molecular and Cellular Biology·Mi-Kyoung KwakThomas W Kensler
Oct 14, 2006·The Journal of Clinical Investigation·Eui-Cheol ShinBarbara Rehermann
Dec 3, 2013·Amino Acids·Xiao-Dong LiuPu-Yan Chen
Mar 20, 2013·Biochimica Et Biophysica Acta·Mary J Kunjappu, Mark Hochstrasser
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Oct 13, 2004·Mutation Research·Mi-Kyoung KwakThomas W Kensler

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