Constitutive c-ets2 expression in M1D+ myeloblast leukemic cells induces their differentiation to macrophages.

Molecular and Cellular Biology
C AperloK E Boulukos

Abstract

The expression of c-ets2 is rapidly induced in a variety of myelomonocytic cell lines as they differentiate into macrophages. We find that constitutive expression of c-ets2 in the M1D+ myeloblast leukemic cell line (M1ets2) is sufficient to push these cells to a more differentiated state. The expression of several differentiation-specific genes is upregulated in M1ets2 cells, including those encoding macrophage-specific lysozyme M and tumor necrosis factor alpha, which are involved in bacteriolytic and inflammatory processes, respectively. Transcription factors c-jun and junB, previously shown to induce partial macrophage differentiation when overexpressed in myelomonocytic leukemia cell lines, are also upregulated in M1ets2 cells. The upregulation of junB is the result of a direct interaction of Ets2 with ets binding sites of the junB promoter, since transient or constitutive Ets2 expression in M1D+ cells activates junB transcription via ets binding sites. In addition, transfection of a dominant negative mutant of Ets2, devoid of its transcriptional activation domain, greatly reduces transcriptional activities of the junB promoter in M1ets2 cells. Finally, unlike their parental M1D+ counterparts, M1ets2 cells secrete the macro...Continue Reading

References

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Citations

Mar 29, 2008·Cell Stem Cell·Stuart M ChambersMargaret A Goodell
Apr 30, 2016·Angiogenesis·Michael P Craig, Saulius Sumanas
Oct 10, 2006·Biochemical and Biophysical Research Communications·Patrick MartinPhilippe Pognonec
Nov 14, 2003·Biology of Reproduction·You-Hong ChengStuart Handwerger
Aug 16, 2006·Oncogene·D-M WangJ S Lipsick
Feb 15, 2001·Oncogene·A Raouf, A Seth
Aug 7, 1999·The Journal of Biological Chemistry·J H KoN Taniguchi
Aug 1, 1997·The Journal of Biological Chemistry·P BuckhaultsM Pierce
Jun 2, 1998·Biochimica Et Biophysica Acta·J Dittmer, A Nordheim
Feb 1, 2003·Gene·Tsuneyuki Oikawa, Toshiyuki Yamada

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