Abstract
The expression of c-ets2 is rapidly induced in a variety of myelomonocytic cell lines as they differentiate into macrophages. We find that constitutive expression of c-ets2 in the M1D+ myeloblast leukemic cell line (M1ets2) is sufficient to push these cells to a more differentiated state. The expression of several differentiation-specific genes is upregulated in M1ets2 cells, including those encoding macrophage-specific lysozyme M and tumor necrosis factor alpha, which are involved in bacteriolytic and inflammatory processes, respectively. Transcription factors c-jun and junB, previously shown to induce partial macrophage differentiation when overexpressed in myelomonocytic leukemia cell lines, are also upregulated in M1ets2 cells. The upregulation of junB is the result of a direct interaction of Ets2 with ets binding sites of the junB promoter, since transient or constitutive Ets2 expression in M1D+ cells activates junB transcription via ets binding sites. In addition, transfection of a dominant negative mutant of Ets2, devoid of its transcriptional activation domain, greatly reduces transcriptional activities of the junB promoter in M1ets2 cells. Finally, unlike their parental M1D+ counterparts, M1ets2 cells secrete the macro...Continue Reading
References
Jul 1, 1978·Cell·M WiglerR Axel
Dec 1, 1979·Virology·T GrafH Beug
Dec 1, 1976·Cell·A Krystosek, L Sachs
Jul 1, 1992·Genes, Chromosomes & Cancer·M Lafage-PochitaloffF Birg
Oct 1, 1991·Molecular and Cellular Biology·P Pognonec, R G Roeder
Jun 1, 1991·Genes & Development·Y Ben-DavidA Bernstein
Apr 20, 1990·Cell·T J SchallH Raab
Mar 1, 1990·Genes & Development·K E BoulukosJ Ghysdael
Jul 1, 1985·Cell·C J SherrE R Stanley
Dec 1, 1989·Molecular and Cellular Biology·K E BoulukosJ Ghysdael
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D K WatsonT S Papas
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J GhysdaelD Stehelin
Nov 1, 1985·Molecular and Cellular Biology·J H Chen
Mar 1, 1987·Journal of Cellular Physiology·C MorganE R Stanley
Dec 4, 1987·Science·L Sachs
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·N K BhatT S Papas
Sep 1, 1981·Virology·C MoscoviciM G Moscovici
Aug 1, 1983·Virology·M G MoscoviciC Moscovici
Nov 24, 1983·Nature·M F NunnP H Duesberg
Nov 24, 1983·Nature·D LeprinceD Stehelin
Aug 16, 1994·Proceedings of the National Academy of Sciences of the United States of America·M T VosoD G Tenen
Jan 29, 1993·Cell·H Q NguyenD A Liebermann
Feb 1, 1995·Molecular and Cellular Biology·S B McMahon, J G Monroe
Dec 1, 1994·The Journal of Experimental Medicine·M A ReddyM C Ostrowski
Sep 9, 1994·Science·E W ScottH Singh
Apr 22, 1994·Cell·T R GolubD G Gilliland
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K ShimizuM Ohki
Feb 1, 1993·Molecular and Cellular Biology·K A LordD A Liebermann
Apr 5, 1996·Cell·M H SiewekeT Graf
Citations
Apr 11, 2008·Experimental Hematology·Ben A CrokerAndrew W Roberts
Jun 15, 2000·Experimental Cell Research·C P VaryA Seth
Mar 29, 2008·Cell Stem Cell·Stuart M ChambersMargaret A Goodell
Mar 3, 2015·Biochimie·Xue-Bin ShenXu Lin
Apr 30, 2016·Angiogenesis·Michael P Craig, Saulius Sumanas
Oct 10, 2006·Biochemical and Biophysical Research Communications·Patrick MartinPhilippe Pognonec
Nov 14, 2003·Biology of Reproduction·You-Hong ChengStuart Handwerger
Aug 16, 2006·Oncogene·D-M WangJ S Lipsick
May 23, 2001·International Immunology·M J ShiJ Stavnezer
May 31, 2002·Oncogene·Yoshiko MaidaMasaki Inoue
Dec 21, 2007·Leukemia·Y GeJ W Taub
Mar 30, 2001·The Journal of Biological Chemistry·L SevillaK E Boulukos
Feb 15, 2001·Oncogene·A Raouf, A Seth
Dec 18, 2002·Blood·Thomas S BushAlan G Rosmarin
Jun 2, 2005·Blood·Toshibumi Shimokawa, Chisei Ra
Aug 7, 1999·The Journal of Biological Chemistry·J H KoN Taniguchi
Apr 29, 2000·The Journal of Biological Chemistry·T YokoiY Murata
Apr 18, 1998·The Journal of Biological Chemistry·I L RossD A Hume
Feb 1, 2000·The Journal of Biological Chemistry·A KimuraY Murata
Mar 17, 1999·Molecular Endocrinology·Y Sun, M L Duckworth
Aug 1, 1997·The Journal of Biological Chemistry·P BuckhaultsM Pierce
Mar 19, 1999·Molecular and Cellular Biology·L SevillaK E Boulukos
Mar 14, 2008·Molecular Cancer Research : MCR·John A HamiltonGlen M Scholz
Oct 12, 2000·Experimental Hematology·G PawlakG Mouchiroud
Jun 2, 1998·Biochimica Et Biophysica Acta·J Dittmer, A Nordheim
Feb 1, 2003·Gene·Tsuneyuki Oikawa, Toshiyuki Yamada