Constitutive differences in Cu/Zn superoxide dismutase mRNA levels and activity in hemocytes of Biomphalaria glabrata (Mollusca) that are either susceptible or resistant to Schistosoma mansoni (Trematoda)

Molecular and Biochemical Parasitology
Cheri P GoodallC J Bayne

Abstract

Genetic strains of the snail Biomphalaria glabrata vary in their resistance to the parasite Schistosoma mansoni. Phagocytic cells (hemocytes) circulating in the hemolymph of B. glabrata play an essential role in the snail's innate immune response. Hemocytes of resistant B. glabrata kill S. mansoni in vitro via a mechanism which involves a respiratory burst. Reactive oxygen species (ROS), which are products of the respiratory burst, can act as mediators of both oxidative damage and of immune-related intracellular signaling pathways. One specific ROS, hydrogen peroxide (H2O2), has been shown to be involved in hemocyte-mediated sporocyst killing. We tested the hypothesis that Cu/Zn superoxide dismutase (SOD), a cytosolic enzyme that catalyzes the conversion of superoxide anion to H2O2, is somehow different between resistant and susceptible snail strains. We report a hemocyte transcript with all the features of a typical cytosolic Cu/Zn SOD (GenBank accession numbers AY505496 and AY505497). The amount of Cu/Zn SOD mRNA in hemocytes from resistant snails was double that of hemocytes from susceptible snails, and this correlated directly with an increased Cu/Zn SOD enzymatic activity in resistant hemocytes. Additional experiments dete...Continue Reading

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Citations

Nov 6, 2007·Developmental and Comparative Immunology·Judith E Humphries, Timothy P Yoshino
Nov 26, 2015·Developmental and Comparative Immunology·E A PilaP C Hanington
Nov 1, 2005·Biochemical and Biophysical Research Communications·Marcelo GonzalezEvelyne Bachère
Aug 6, 2005·Journal of Invertebrate Pathology·Younghun JungCoen M Adema
Aug 19, 2015·Developmental and Comparative Immunology·Judith Humphries, Briana Harter
May 15, 2009·Molecular and Biochemical Parasitology·Matty KnightChristopher J Bayne
Nov 25, 2019·Developmental and Comparative Immunology·Maria G CastilloCesar E Montelongo

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