Constitutive expression of antimicrobial peptide PR-39 in transgenic mice significantly enhances resistance to bacterial infection and promotes growth

Transgenic Research
Fang ZengZicong Li

Abstract

Use of huge amounts of antibiotics in farm animal production has promoted the prevalence of antibiotic-resistant bacteria, which poses a serious threat to public health. Therefore, alternative approaches are needed to reduce or replace antibiotic usage in the food animal industry. PR-39 is a pig-derived proline-rich antimicrobial peptide that has a broad spectrum of antibacterial activity and a low propensity for development of resistance by microorganisms. To test whether ubiquitous expression of PR-39 in transgenic (TG) mice can increase resistance against bacterial infection, we generated TG mice that ubiquitously express a pig-derived antimicrobial peptide PR-39 and analyzed their growth and resistance to infection of the highly pathogenic Actinobacillus pleuropneumoniae (APP) isolated from swine. The growth performance was significantly increased in TG mice compared with their wild-type (WT) littermates. After the APP challenge, TG mice exhibited a significantly higher survival rate and significantly lower tissue bacterial load than WT littermates. Furthermore, the tissue lesion severity that resulted from APP infection was milder in TG mice than that in their WT littermates. This study provides a good foundation for the d...Continue Reading

References

Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·G H GudmundssonH G Boman
Nov 15, 1993·Biochemical and Biophysical Research Communications·P Storici, M Zanetti
Mar 23, 2002·Microbes and Infection·Balaji RamanathanFrank Blecha
Feb 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Phillip H A LeeRichard L Gallo
Mar 10, 2005·Basic & Clinical Pharmacology & Toxicology·Frank M Aarestrup
Dec 13, 2006·Nature Biotechnology·Robert E W Hancock, Hans-Georg Sahl
Mar 5, 2008·Antimicrobial Agents and Chemotherapy·Queenie C K CheungJulang Li
Jun 27, 2008·Developmental and Comparative Immunology·Yongming Sang, Frank Blecha
Jan 16, 2014·Research in Veterinary Science·Joanne L HardstaffPiran C L White
Apr 24, 2014·PloS One·Edwin J A VeldhuizenHenk P Haagsman
Mar 21, 2015·Proceedings of the National Academy of Sciences of the United States of America·Thomas P Van BoeckelRamanan Laxminarayan
May 29, 2015·Journal of Animal Science and Biotechnology·Hao XiaoYulong Yin
May 7, 2016·International Journal of Molecular Sciences·Shuai WangShiyan Qiao

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
transfection
PCR
biopsies
fluorescence microscopy
ELISA

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Alzheimer's Disease: APP

Amyloid precursor protein (APP) proteolysis is critical for the development of Alzheimer's disease, a neurodegenerative disease associated with accumulation of amyloid plaques in the brain. Here is the latest research on APP and Alzheimer's disease.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Related Papers

Antimicrobial Agents and Chemotherapy
Queenie C K CheungJulang Li
Journal of the Science of Food and Agriculture
Gee Leng LauYin Wan Ho
Medicinski pregled
Sonja VuckovićRadan Stojanović
Indian Journal of Community Medicine : Official Publication of Indian Association of Preventive & Social Medicine
Shubra PooniaDechen C Tsering
© 2022 Meta ULC. All rights reserved