PMID: 8592086Jan 1, 1996Paper

Constitutive expression of both subunits of interleukin-12 in human keratinocytes

The Journal of Investigative Dermatology
N YawalkarL R Braathen

Abstract

Interleukin-12 (IL-12), a heterodimeric molecule consisting of disulfide-linked 35- and 40-kDa chains, is secreted by a variety of cells including macrophages and B cells. While keratinocytes have recently been demonstrated to produce IL-12 after stimulation with phorbol-12,13-dibutyrate as well as trinitrochlorobenzene, the constitutive expression of the two IL-12 subunits has remained controversial. In this study, we investigate if cultured keratinocytes derived from human epidermis and the follicle outer root sheath constitutively express IL-12. Total RNA was reverse transcribed to cDNA and amplified using a highly sensitive nested reverse transcriptase polymerase chain reaction. Both IL-12 p40 and p35 transcripts were detected in keratinocyte cultures. Moreover, low levels of the IL-12 p70 heterodimer were detected in the culture supernatants, as determined by a sensitive enzyme-linked immunosorbent assay. Since IL-12 is known to play an important role in the development of Th1 cells, the constitutive expression of mRNA for IL-12 in keratinocytes together with its secretion adds further evidence for a role of keratinocytes in immunological processes within the skin such as in contact hypersensitivity.

Citations

Mar 20, 1998·International Reviews of Immunology·G Trinchieri
May 14, 2005·Cancer Immunology, Immunotherapy : CII·Hui LiAiping H Young
Jan 8, 1998·Seminars in Cutaneous Medicine and Surgery·L R Eversole
Jan 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gamze PiskinMarcel B M Teunissen
Jan 24, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kei-ichi YamanakaThomas S Kupper
Jul 31, 1998·Clinical Immunology and Immunopathology·R R Caspi

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