Constitutive expression of inducible nitric oxide synthase in human bronchial epithelial cells induces c-fos and stimulates the cGMP pathway

American Journal of Respiratory Cell and Molecular Biology
E Felley-BoscoC C Harris

Abstract

Two major roles have been defined for nitric oxide (NO): cell-cell communication mediated by the stimulation of cyclic guanosine 3',5'-monophosphate (cGMP) synthesis and cytotoxicity by direct or indirect interaction of the free radical NO with cellular targets. Thus, pathologic states might result from an alteration of NO pathways, e.g., by deregulated activity of NO synthase. To investigate this hypothesis, we introduced the murine-inducible NO synthase (iNOS) sequence into immortalized human bronchial epithelial cells (BEAS-2B). iNOS activity, measured by conversion of [14C]arginine to [14C]citrulline in the presence of 1 mM EGTA, was higher than 100 pmol/min/mg protein in early passages of iNOS-transfected cells but decreased with cell subculturing. No iNOS activity could be detected in control vector-transfected cells. NO stimulated cGMP production in iNOS-transfected cells, and this effect was inhibited by the iNOS inhibitor NG-monomethyl-L-arginine. In addition, NO production induced c-fos expression and did not interfere with clonal cell growth. These results suggest that BEAS-2B cells constitute a suitable model to study the consequences of iNOS activity on signal transduction pathways in bronchial epithelium.

Citations

Nov 28, 2002·International Journal of Cancer. Journal International Du Cancer·Yoshiyuki IshiiHiroyasu Esumi
Nov 1, 1995·Research in Immunology·P J Barnes
Feb 13, 2001·European Journal of Pharmacology·D BredenbrökerU Wagner
Apr 1, 1997·British Journal of Pharmacology·S P RangeA J Knox
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·F H GuoS C Erzurum
May 1, 1998·American Journal of Respiratory and Critical Care Medicine·X Y LiW MacNee
Dec 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·E Felley-BoscoA F Quest
Dec 1, 1994·International Journal of Cancer. Journal International Du Cancer·J MurataL Juillerat-Jeanneret
Dec 1, 1996·Kidney International·S D SmithR M Weiss
Jan 1, 1997·The Journal of Allergy and Clinical Immunology·S A KharitonovP J Barnes
May 1, 1998·The Journal of Allergy and Clinical Immunology·P J Barnes
May 10, 2011·Nitric Oxide : Biology and Chemistry·Jingjing Jiang, Steven C George
Dec 4, 2014·Acta histochemica·Armanda PugnaloniAlessandro F Gualtieri
Mar 10, 1999·Respiratory Medicine·M K al-AliP H Howarth
Mar 13, 2009·PloS One·Jérôme RambertM Djavad Mossalayi
Dec 13, 2003·Biological Chemistry·Klaus-Dietrich Kröncke
Jun 26, 2002·The Journal of Biological Chemistry·Darren E CasteelRenate B Pilz
Jul 2, 2003·FEBS Letters·Muriel André, Emanuela Felley-Bosco
Oct 12, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·H E MarshallJ S Stamler
Mar 1, 1996·Thorax·P J Barnes, S A Kharitonov
Jun 25, 1998·The American Journal of Physiology·D C ThompsonA M Malkinson
Jul 17, 1999·The American Journal of Physiology·T JillingS Matalon
Dec 31, 1997·The American Journal of Physiology·C L HartsfieldA M Choi
Sep 18, 1997·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·B C Kone

❮ Previous
Next ❯

Related Concepts

Related Feeds

American Thoracic Association Journals

Discover the latest respiratory research published by the journals from the American Thoracic Society.