PMID: 6972533Jan 1, 1981Paper

Constitutive gene expression in myeloid leukemia and cell competence for induction of differentiation by the steroid dexamethasone

Proceedings of the National Academy of Sciences of the United States of America
L Cohen, L Sachs

Abstract

Regulation of the cytoplasmic protein changes during myeloid cell differentiation has been analyzed with two-dimensional gel electrophoresis and differentiation-defective cell mutants. The cells studied include a clone of myeloid leukemia cells (clone 11) that can be induced to differentiate to macrophages by the protein inducer MGI and the steroid dexamethasone (Dex) and mutant clones that were inducible for differentiation to macrophages by MGI but not by Dex. The mutants were not defective in the specific binding of [3H]Dex to cytoplasmic receptors or in the transport and nuclear binding of the receptor--steroid complex. The protein patterns in the mutants showed both specific constitutive protein changes and nonresponding proteins. Twenty-one percent of the Dex-induced protein changes and 2% of the MGI-induced protein changes in clone 11 were constitutively expressed in the mutants. In addition, 28% of the proteins that responded to Dex in clone 11 did not respond to Dex in the mutants, whereas only 4% of the proteins that responded to MGI in clone 11 did not respond to MGI. The higher percentage of constitutive changes was thus associated with a larger defect in induction. The proteins with an abnormal response to Dex stil...Continue Reading

References

Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P Linser, A A Moscona
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J Lotem, L Sachs
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J Lotem, L Sachs
Jan 1, 1978·Annual Review of Biochemistry·P A Marks, R A Rifkind
Sep 1, 1977·Journal of Cellular Physiology·A Krystosek, L Sachs
May 15, 1975·International Journal of Cancer. Journal International Du Cancer·J Lotem, L Sachs
Aug 15, 1975·European Journal of Biochemistry·R A Laskey, A D Mills
Feb 1, 1974·Journal of Cellular Physiology·B A Sela, L Sachs
Aug 1, 1974·Cell·C H Sibley, G M Tomkins
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J Lotem, L Sachs
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey

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Citations

Aug 23, 2002·Seminars in Cancer Biology·Joseph Lotem, Leo Sachs
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·L Sachs
Apr 1, 1994·Leukemia & Lymphoma·G J KaspersA J Veerman
Jan 29, 2014·Proceedings of the National Academy of Sciences of the United States of America·Michael Sela, Yoram Groner
Jan 1, 1986·Leukemia Research·P Sentein, D Le Coq
Aug 21, 1987·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·L Sachs
Aug 1, 1982·Journal of Cellular Physiology·N A Nicola, D Metcalf
Apr 1, 1982·Journal of Cellular Physiology·G Symonds, L Sachs
Dec 1, 1986·Cell Biophysics·L Sachs
Dec 15, 1983·International Journal of Cancer. Journal International Du Cancer·J Lotem, L Sachs
Nov 13, 2014·Molecular Biology Reports·Ernesto Burgio, Lucia Migliore
Jan 1, 1985·Critical Reviews in Oncology/hematology·R Taetle, J A Koziol

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