Constitutive nitric oxide production by rat alveolar macrophages

The American Journal of Physiology
P R MilesL Huffman

Abstract

Results from previous studies suggest that alveolar macrophages must be exposed to inflammatory stimuli to produce nitric oxide (.NO). In this study, we report that naive unstimulated rat alveolar macrophages do produce .NO and attempt to characterize this process. Western blot analysis demonstrates that the enzyme responsible is an endothelial nitric oxide synthase (eNOS). No brain or inducible NOS can be detected. The rate of .NO production is approximately 0.07 nmol.10(6) cells-1.h-1, an amount that is less than that produced by the eNOS found in alveolar type II or endothelial cells. Alveolar macrophage .NO formation is increased in the presence of extracellular L-arginine, incubation medium containing magnesium and no calcium, a calcium ionophore (A-23187), or methacholine. .NO production is inhibited by NG-nitro-L-arginine methyl ester (L-NAME) but not by NG-nitro-L-arginine, L-N5-(1-iminomethyl)ornithine hydrochloride, or aminoguanidine. Incubation with ATP, ADP, or histamine also inhibits .NO formation. Some of these properties are similar to and some are different from properties of eNOS in other cell types. Cellular .NO levels do not appear to be related to ATP or lactate content. Alveolar macrophage production of .NO...Continue Reading

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Citations

Mar 8, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·J SiroisE Y Bissonnette
Dec 26, 2001·American Journal of Respiratory Cell and Molecular Biology·Susan A McDowellSusan E Waltz
Dec 8, 2010·Infection and Immunity·Allan Jefferson GuimarãesJoshua Daniel Nosanchuk
May 19, 2009·American Journal of Respiratory Cell and Molecular Biology·Lester Kobzik
Jun 22, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kazutoshi HamanakaJames C Parker
Oct 6, 2000·American Journal of Respiratory Cell and Molecular Biology·G Ménard, E Y Bissonnette
Mar 11, 2000·American Journal of Physiology. Cell Physiology·A Nishiyama-Naruke, R Curi
Aug 6, 2021·Frontiers in Cellular and Infection Microbiology·Amy A O'CallaghanSinéad C Corr
May 23, 2020·Current Medicinal Chemistry·Zsófia LázárAndras Bikov

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