Constitutively active STAT5b induces cytokine-independent growth of the acute myeloid leukemia-derived MUTZ-3 cell line and accelerates its differentiation into mature dendritic cells

Journal of Immunotherapy
Hetty J BontkesErik Hooijberg

Abstract

The CD34(+) human acute myeloid leukemia-derived cell line MUTZ-3 is dependent on hematopoietic growth factors for its proliferation and is able to differentiate into dendritic cells (DCs) in response to the combination of granulocyte-macrophage colony-stimulating factor, interleukin-4, and tumor necrosis factor-alpha. This cell line carries human leukocyte antigen (HLA)-A2.1, HLA-A3, and HLA-B44, which cover most of the caucasian population, and it could therefore be used as an off-the-shelf allogeneic DC-based vaccine. Signal transduction and activation of transcription (STAT) 5b is involved in cytokine signal transduction, particularly of cytokines involved in DC precursor growth and differentiation. The constitutively active form of STAT5b induced cytokine-independent growth of MUTZ-3 cells. Furthermore, STAT5b-transduced cells differentiated into mature DCs in 3 to 4 days after stimulation with DC differentiation-inducing cytokines, reducing the culture period to obtain mature DCs with 5 days compared with unmodified MUTZ-3-derived mature DC cultures. Both DC types expressed DC maturation markers and were equally effective in inducing primary T-cell responses. DCs derived from the STAT5b-transduced cells had a more stable ...Continue Reading

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Citations

Jun 22, 2013·Journal of Translational Medicine·Annelies W TurksmaErik Hooijberg
Dec 22, 2012·Immunotherapy·Annelies W TurksmaErik Hooijberg
Dec 22, 2011·Journal of Biomedicine & Biotechnology·Jian NingDerwood Pamphilon
Jul 7, 2007·British Journal of Haematology·Jane RasaiyaahBenjamin M Chain
Aug 15, 2014·Frontiers in Physiology·Dimitry A ChistiakovYuri V Bobryshev
Aug 19, 2009·PloS One·Yui HaradaYoshikazu Yonemitsu
Mar 1, 2012·Cellular and Molecular Bioengineering·Nripen S SharmaMartin L Yarmush
Jul 31, 2008·Journal of Leukocyte Biology·Saskia J A M SantegoetsTanja D de Gruijl
Aug 8, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·María MorenoHetty J Bontkes
May 24, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lianne van de LaarAndrea M Woltman
Aug 21, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lydon Wainaina NyamburaPeter Walden
Mar 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Saskia J A M SantegoetsTanja D de Gruijl
Feb 14, 2018·Bioconjugate Chemistry·Alexandra N Tsoras, Julie A Champion

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