Constitutively overexpressed 21 kDa protein in Hodgkin lymphoma and aggressive non-Hodgkin lymphomas identified as cytochrome B5b (CYB5B).

Molecular Cancer
Derek MurphyDiponkar Banerjee

Abstract

We have previously reported a novel constitutively overexpressed 21 kDa protein in Hodgkin Lymphoma (HL) and aggressive Non-Hodgkin Lymphomas (NHL). The objective of the current study was to 1) identify this protein using two independent methods, 2) study the expression of the protein and its encoding mRNA in reactive lymph nodes, normal lymphocytes and CD34+ bone marrow precursor cells, 3) analyse patterns of expression of the protein in tissue microarrays assembled from a large number of diagnostic clinical biopsies from patients with HL, and 4) determine the copy number variation and mutation status of the encoding gene in HL cell lines. Peptide sequencing by LC-MS/MS and protein identification by protein array screening identified a single protein, CYB5B. No mutations were detected in the CYB5B gene in HL cell lines. Quantitative PCR showed CYB5B gene expression was increased in HL and NHL cell lines. Array CGH using a submegabase resolution tiling array revealed gains in the CYB5B locus in HL cell lines KMH2 and L428. Membrane expression was seen in Reed-Sternberg cells in clinical biopsies from patients with HL but not in reactive lymph nodes. Bone marrow CD34+ precursor cells were CYB5B negative on the cell surface. RT-P...Continue Reading

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Citations

Sep 20, 2015·Cellular Oncology (Dordrecht)·R N SharanMandahakani Ksoo
Jun 8, 2017·Future Oncology·Pui Chi TsuiVivian Wai Yan Lui
Jun 29, 2012·Journal of Vascular Research·Ward De SpiegelaerePieter Cornillie

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Datasets Mentioned

BETA
SR786
AK291576

Methods Mentioned

BETA
electrophoresis
protein array
PCR
confocal microscopy
biopsies
flow cytometry
PCRs
density gradient centrifugation
FCS
Flow Cytometric

Software Mentioned

Ensembl
R
VisualGrid
Imagenes
7900 HT - system sequence detection systems ( SDS )
SeeGH
Bioconductor
SoftWoRx Tracker Spot Analysis
FCS Express

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