Constraints on supramolecular structure in amyloid fibrils from two-dimensional solid-state NMR spectroscopy with uniform isotopic labeling

Journal of the American Chemical Society
Robert Tycko, Yoshitaka Ishii

Abstract

We show that strong constraints on supramolecular structure in amyloid fibrils can be obtained from solid-state nuclear magnetic resonance measurements on samples with uniformly 13C-labeled segments. The measurements exploit two-dimensional (2D) 13C-13C exchange spectroscopy in conjunction with high-speed magic angle spinning, with proton-mediated exchange of 13C nuclear spin magnetization as recently demonstrated by Baldus and co-workers (J. Am. Chem. Soc. 2002, 124, 9704-9705). Proton-mediated 2D exchange spectra of fibrils formed by residues 16-22 of the 40-residue Alzheimer's beta-amyloid peptide show strong nonsequential, intermolecular cross-peaks between alpha-carbons that dictate an antiparallel beta-sheet structure in which residue 16+k aligns with residue 22-k. The strong alpha/alpha cross-peaks are absent from conventional, direct 2D exchange spectra. Proton-mediated 2D exchange spectra of fibrils formed by residues 11-25 indicate an antiparallel beta-sheet structure with a pH-dependent intermolecular alignment. In contrast, proton-mediated 2D exchange spectra of fibrils formed by the full-length beta-amyloid peptide are consistent with a parallel beta-sheet structure. These data show that the supramolecular structur...Continue Reading

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Citations

Jun 2, 2005·Journal of Biomolecular NMR·Kerstin RiedelRamadurai Ramachandran
Jun 3, 2008·Journal of Biomolecular NMR·Christian HerbstRamadurai Ramachandran
Aug 9, 2012·Journal of Biomolecular NMR·Ivano BertiniEnrico Ravera
Nov 19, 2013·Journal of Biomolecular NMR·Lucio FerellaAntonio Rosato
Apr 23, 2004·Current Opinion in Structural Biology·Robert Tycko
Aug 12, 2004·Chemical Reviews·Stanley J Opella, Francesca M Marassi
May 30, 2006·The Journal of Chemical Physics·Anant K Paravastu, Robert Tycko
Mar 10, 2012·Proceedings of the National Academy of Sciences of the United States of America·Wei QiangRobert Tycko
Jun 21, 2011·Bioinformatics·Charles W O'DonnellBonnie Berger
Aug 13, 2004·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Suzana K Straus
Dec 13, 2005·The Journal of Clinical Investigation·Yona LevitesTodd E Golde
Sep 18, 2012·The Journal of Chemical Physics·Wei Qiang, Robert Tycko
Jan 7, 2009·The Journal of Chemical Physics·Gaël De PaëpeRobert G Griffin
Jan 13, 2004·Proceedings of the National Academy of Sciences of the United States of America·Christopher P JaroniecRobert G Griffin
Apr 23, 2014·Protein Expression and Purification·Iga KosickaNiels Chr Nielsen
Dec 3, 2014·Progress in Nuclear Magnetic Resonance Spectroscopy·Shenlin Wang, Vladimir Ladizhansky
Jun 15, 2010·Biophysical Chemistry·Kan-Nian Hu, Robert Tycko
Jul 30, 2010·Protein Science : a Publication of the Protein Society·Jun-Xia LuRobert Tycko
Feb 12, 2005·Journal of Magnetic Resonance·John A StringerChad M Rienstra
Feb 10, 2006·Nanomedicine : Nanotechnology, Biology, and Medicine·Alexey V KransnoslobodtsevYuri L Lyubchenko
Nov 18, 2005·Journal of Molecular Biology·Chad McAllisterYuri L Lyubchenko
Oct 11, 2005·Journal of Molecular Biology·Nicolae-Viorel BucheteGerhard Hummer
May 11, 2005·Journal of Molecular Biology·Manuela López de la PazGiorgio Colombo
Jun 30, 2016·Journal of the American Chemical Society·Michael T ColvinRobert G Griffin
May 17, 2017·Current Opinion in Endocrinology, Diabetes, and Obesity·Nischay K RegeMichael A Weiss
Jul 21, 2006·Angewandte Chemie·Kerstin RiedelRamadurai Ramachandran
Dec 29, 2007·Chembiochem : a European Journal of Chemical Biology·Henrike Heise
Jun 6, 2008·Angewandte Chemie·René VerelBeat H Meier
Apr 11, 2014·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Lucia BanciEnrico Ravera

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