Constructing a Robust Fluorescent DNA-Stabilized Silver Nanocluster Probe Module by Attaching a Duplex Moiety

Chemistry : a European Journal
Ruoyun LinNa Li

Abstract

Fluorescent DNA-templated silver nanoclusters (DNA-Ag NCs) have served as excellent luminescent probes and operation units in various applications. However, the fluorescence property of DNA-Ag NCs is very sensitive to elongation or modification of the DNA template, limiting the breadth of applications. In this work, we propose a strategy for constructing a robust fluorescent DNA-Ag NCs probe module by attaching a duplex moiety to the nanocluster-bearing sequence. The fluorescence intensity of the DNA-Ag NCs can be enhanced 90-fold upon hybridization of the elongated moiety. Adenine in the linker sequence has a further enhancing effect on the fluorescence intensity, whereas thymine has a quenching effect. The transformation from a non-fluorescent species to fluorescent nanoclusters is responsible for the fluorescence enhancement with duplex formation of the elongated moiety. We hope that this design will aid future diversification of experimental designs to facilitate more applications that are currently limited by the aforementioned problems.

References

Jan 6, 2001·Science·L A PeyserR M Dickson
Nov 21, 2002·Journal of the American Chemical Society·Jie Zheng, Robert M Dickson
Apr 22, 2004·Journal of the American Chemical Society·Jeffrey T PettyRobert M Dickson
Nov 16, 2006·Annual Review of Physical Chemistry·Jie ZhengRobert M Dickson
Dec 13, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Matthew R BranhamRoyce W Murray
Mar 19, 2008·Journal of the American Chemical Society·Chris I RichardsRobert M Dickson
Jul 26, 2008·Nature Nanotechnology·Lorenzo Berti, Glenn A Burley
Jan 11, 2007·The Journal of Physical Chemistry. C, Nanomaterials and Interfaces·Caroline M RitchieJeffrey T Petty
Apr 20, 2010·Advanced Materials·Hangxun Xu, Kenneth S Suslick
Aug 12, 2010·Nano Letters·Hsin-Chih YehJames H Werner
Dec 1, 2010·The Journal of Physical Chemistry Letters·Jeffrey T PettyRobert M Dickson
Dec 15, 2010·Chemical Communications : Chem Comm·Jaswinder SharmaJennifer S Martinez
Mar 17, 2011·Nanoscale·Isabel Díez, Robin H A Ras
May 17, 2011·The Journal of Physical Chemistry. B·Jeffrey T PettyRobert M Dickson
Aug 23, 2011·Analytical and Bioanalytical Chemistry·Bingyan Han, Erkang Wang
Nov 15, 2011·Chemical Society Reviews·Sungmoon ChoiJunhua Yu
Nov 22, 2011·Analytical Chemistry·Jeffrey T PettyBidisha Sengupta
Apr 11, 2012·Chemistry : a European Journal·Zhenzhen HuangXiaogang Qu
Jun 23, 2012·Accounts of Chemical Research·Huifeng QianRongchao Jin
Jul 11, 2012·Journal of the American Chemical Society·Hsin-Chih YehJames H Werner
Jun 8, 2013·The Journal of Physical Chemistry Letters·Jeffrey T PettyRobert M Dickson
Jul 6, 2013·Nanoscale·Judy M OblioscaHsin-Chih Yeh
Mar 20, 2014·Nanotechnology·Sylwia WalczakJuewen Liu
Sep 25, 2015·Chemical Society Reviews·Yu TaoXiaogang Qu
Jan 1, 2015·The Journal of Physical Chemistry. C, Nanomaterials and Interfaces·Mainak GangulyJeffrey T Petty

❮ Previous
Next ❯

Citations

May 10, 2019·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Shiyin WengManzhou Zhu
May 4, 2020·Journal of Photochemistry and Photobiology. B, Biology·Sajena Kanangat SaraswathiJoshy Joseph
Sep 1, 2021·Journal of the American Chemical Society·Qiong WuWeihong Tan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.