Construction and analysis of an infectious human Immunodeficiency virus type 1 subtype C molecular clone

Journal of Virology
T Ndung'uM Essex

Abstract

Human immunodeficiency virus type 1 (HIV-1) subtype C is now the predominant subtype in the global epidemic. This subtype is encountered in southern Africa and parts of Asia, where the epidemic is rapidly spreading. One possible explanation for these epidemiological observations is that this subtype has genetic characteristics that may contribute to its spread and/or pathogenic potential. In this report, we describe the construction of MJ4, an infectious chimeric molecular clone of HIV-1 subtype C that replicates in donor peripheral blood mononuclear cells and macrophages. We also tested this clone for its ability to use the chemokine receptors CCR1, CCR2b, CCR3, CXCR4, and CCR5 and found that the clone utilizes only CCR5 as the coreceptor for cell entry. The MJ4 clone will be useful in further biological and virological characterization of HIV-1 subtype C and will be an important tool in the continuing efforts to understand what may constitute protective immunity in HIV-1. The clone may also be used in experimental design of vaccine candidates that may be directed against HIV-1 subtype C.

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Citations

Feb 1, 2007·AIDS Research and Human Retroviruses·Joana P MonteiroBernardo Galvão-Castro
Dec 13, 2005·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Irene N KoulinskaMax Essex
Aug 30, 2008·Journal of Virology·Angsana PhuphuakratPrasert Auewarakul
Jan 13, 2012·Journal of Virology·Jaclyn K WrightThumbi Ndung'u
May 31, 2014·PLoS Pathogens·Amit SharmaMamuka Kvaratskhelia
Jun 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Mario P S ChinWei-Shau Hu
Jul 30, 2014·Molecular Therapy. Nucleic Acids·Robert J ScarboroughAnne Gatignol
May 17, 2006·Journal of Virological Methods·Christine M RousseauJames I Mullins
Jan 13, 2006·Virology·Thumbi Ndung'uMax Essex

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