Jul 22, 1998

Construction and analysis of luxCDABE-based plasmid sensors for investigating N-acyl homoserine lactone-mediated quorum sensing

FEMS Microbiology Letters
M K WinsonG S Stewart

Abstract

Plasmid reporter vectors have been constructed which respond to activation of LuxR and its homologues LasR and RhlR (VsmR) by N-acyl homoserine lactones (AHLs). The expression of luxCDABE from transcriptional fusions to PluxI, PlasI and PrhlI respectively, occurs in the presence of activating AHLs. A profile of structure/activity relationships is seen where the natural ligand is most potent. The characterisation of individual LuxR homologue/AHL combinations allows a comprehensive evaluation of quorum sensing signals from a test organism.

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Mentioned in this Paper

Gene Expression Regulation, Bacterial
Shuttle Vectors
Conjugation
Bacterial Proteins
Alkalescens-Dispar Group
Agar
Agrobacterium tumefaciens
Structure-Activity Relationship
Biosensors
Homoserine lactone, (S)-isomer

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