PMID: 375200Mar 1, 1979Paper

Construction and analysis of recombinant DNAs containing a structural gene for rat prolactin

Nucleic Acids Research
E J GubbinsJ E Donelson

Abstract

Poly A-containing RNA enriched in prolactin-coding sequences was isolated from female rate pituitaries after induction with diethylstilbesterol. Double stranded cDNA was synthesized from this RNA and inserted into plasmid pBR322 at the Pst I site via the poly(dG):polyy(dC) tailing method. E. coli was transformed with this DNA and the recombinant plasmid in one of the transformants characterized in detail. About half of its 900 base pair cDNA insert was sequenced. The DNA sequence is consistent with most of the reported amino acid sequence of rat preprolactin. In addition, the recombinant plasmids in two of the other transformants appear to contain growth hormone coding sequences.

References

Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A M Maxam, W Gilbert
May 1, 1977·Gene·E J GubbinsJ E Donelson
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·L Villa-KomaroffW Gilbert
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R P Ambler, G K Scott
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J G Sutcliffe
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·G A EvansM G Rosenfeld
Nov 28, 1978·Biochemistry·D J McKean, R A Maurer
Oct 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M Grunstein, D S Hogness
Jun 4, 1974·Biochemistry·V GlisinC Byus
Jun 13, 1966·Biochemical and Biophysical Research Communications·D T Denhardt

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Citations

Aug 10, 2006·Mammalian Genome : Official Journal of the International Mammalian Genome Society·S M Khorshed AlamMichael J Soares
Jan 22, 2008·Journal of Mammary Gland Biology and Neoplasia·Josephine F TrottRussell C Hovey
Oct 1, 1986·Molecular and Cellular Endocrinology·J F Amara, P S Dannies
Sep 1, 1991·Molecular and Cellular Endocrinology·G R WilliamsM C Sheppard
Nov 1, 1993·The Journal of Steroid Biochemistry and Molecular Biology·C B WhorwoodP M Stewart
Feb 13, 2001·Pharmacology, Biochemistry, and Behavior·J R DaveG J Kant
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D I Linzer, D Nathans
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P C HiestandC Permmongkol
Jan 11, 1980·Nucleic Acids Research·R GranthamA Pavé
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·T Y ChunJ Gorski
Jan 1, 1987·Developmental Genetics·R T Stone, R A Maurer
Sep 26, 2013·Molecular and Cellular Endocrinology·Y Sangeeta Devi, Julia Halperin
Dec 19, 2002·Endocrinology·Dustin O WiemersMichael J Soares
Jan 1, 1983·CRC Critical Reviews in Biochemistry·A C PaladiniE Poskus

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