Construction and application of a zinc-specific biosensor for assessing the immobilization and bioavailability of zinc in different soils

Environmental Pollution
Pulin LiuWenli Chen

Abstract

The inducibility and specificity of different czcRS operons in Pseudomonas putida X4 were studied by lacZ gene fusions. The data of β-glycosidase activity confirmed that the czcR3 promoter responded quantitatively to zinc. A zinc-specific biosensor, P. putida X4 (pczcR3GFP), was constructed by fusing a promoterless enhanced green fluorescent protein (egfp) gene with the czcR3 promoter in the chromosome of P. putida X4. In water extracts of four different soils amended with zinc, the reporter strain detected about 90% of the zinc content of the samples. Both the bioavailability assessment and the sequential extraction analysis demonstrated that the immobilization of zinc was highly dependent on the physico-chemical properties of soils. The results also showed that the lability of zinc decreased over time. It is concluded that the biosensor constitutes an alternative system for the convenient evaluation of zinc toxicity in the environment.

References

Nov 22, 2002·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Barry P Rosen
Jun 28, 2003·FEMS Microbiology Reviews·Dayle K Blencowe, Andrew P Morby
Aug 14, 2003·Ecotoxicology and Environmental Safety·Christopher Rensing, Raina M Maier
Dec 4, 2003·Environmental Microbiology·David CánovasVíctor de Lorenzo
Sep 4, 2004·Environmental Microbiology·Jan Roelof van der MeerMarco Jaspers
Jan 10, 2006·Environmental Toxicology and Chemistry·Anne KahruHenri-Charles Dubourguier
Apr 26, 2008·Applied Microbiology and Biotechnology·Ya-Juan FuQiao-Yun Huang
Jul 28, 2009·Environmental Pollution·Erica DonnerStephen Nortcliff
Apr 10, 2010·Applied Microbiology and Biotechnology·He WeiLin Xian-Gui
Dec 24, 2010·Journal of Environmental Sciences (China)·Mariavittoria Zampella, Paola Adamo

❮ Previous
Next ❯

Citations

Jan 19, 2016·The Science of the Total Environment·Youngdae YoonYoun-Joo An
May 13, 2014·The Science of the Total Environment·Stéphanie Sayen, Emmanuel Guillon
Dec 1, 2017·Analytical and Bioanalytical Chemistry·Hyun Ju KimSang Jun Lee
Feb 27, 2021·Frontiers in Microbiology·Ilenne Del ValleLauren B Stadler
Mar 13, 2021·Frontiers in Bioengineering and Biotechnology·Ni ZengPeng Cai
Apr 9, 2021·Advanced Materials·Heyu YinChangyong Cao
Jun 8, 2021·Biosensors & Bioelectronics·Michael MoraskieSylvia Daunert
Oct 10, 2014·ACS Synthetic Biology·Lara Tess Bereza-MalcolmAshley Edwin Franks

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.