Construction and applications of DNA probes for detection of polychlorinated biphenyl-degrading genotypes in toxic organic-contaminated soil environments.

Applied and Environmental Microbiology
S WaliaN Rosenthal

Abstract

Several DNA probes for polychlorinated biphenyl (PCB)-degrading genotypes were constructed from PCB-degrading bacteria. These laboratory-engineered DNA probes were used for the detection, enumeration, and isolation of specific bacteria degrading PCBs. Dot blot analysis of purified DNA from toxic organic chemical-contaminated soil bacterial communities showed positive DNA-DNA hybridization with a 32P-labeled DNA probe (pAW6194, cbpABCD). Less than 1% of bacterial colonies isolated from garden topsoil and greater than 80% of bacteria isolated from PCB-contaminated soils showed DNA homologies with 32P-labeled DNA probes. Some of the PCB-degrading bacterial isolates detected by the DNA probe method did not show biphenyl clearance. The DNA probe method was found to detect additional organisms with greater genetic potential to degrade PCBs than the biphenyl clearance method did. Results from this study demonstrate the usefulness of DNA probes in detecting specific PCB-degrading bacteria, abundance of PCB-degrading genotypes, and genotypic diversity among PCB-degrading bacteria in toxic chemical-polluted soil environments. We suggest that the DNA probe should be used with caution for accurate assessment of PCB-degradative capacity wit...Continue Reading

References

Apr 1, 1989·Applied and Environmental Microbiology·A Khan, S Walia
Oct 1, 1985·Journal of Clinical Microbiology·F A RubinL S Baron
May 1, 1987·Applied and Environmental Microbiology·D L BedardM J Brennan
May 1, 1987·Applied and Environmental Microbiology·R K JainD Pacia
Feb 1, 1988·Applied and Environmental Microbiology·M R Barton, R L Crawford
Nov 1, 1983·Applied and Environmental Microbiology·R FittsM Neri
Sep 1, 1983·Applied and Environmental Microbiology·W E HillC C Aulisio
Feb 1, 1988·Applied and Environmental Microbiology·H A PetrickW H Holzapfel
Mar 1, 1988·Applied and Environmental Microbiology·William E HolbenJames M Tiedje
Oct 1, 1988·Applied and Environmental Microbiology·P SimonetR Bardin

❮ Previous
Next ❯

Citations

Dec 1, 1994·Biodegradation·V BrennerD D Focht
Jun 6, 2003·Applied and Environmental Microbiology·Brett R BaldwinLoring Nies
May 2, 1994·Annals of the New York Academy of Sciences·A C LaytonG S Sayler
Jun 10, 2000·Journal of Applied Microbiology·L CavalcaV Andreoni
Oct 20, 1999·Critical Reviews in Biotechnology·B K SinghK K Tripathi
Jun 26, 2002·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Satish WaliaRasul Chaudhry
Oct 1, 1991·The Journal of Applied Bacteriology·P S CocconcelliV Bottazzi
Jan 1, 1996·Applied Biochemistry and Biotechnology·D P Chandler, F J Brockman
Jan 1, 1996·Critical Reviews in Microbiology·S KumarR Lal
Aug 4, 1998·Applied and Environmental Microbiology·I Wagner-DöblerE R Moore
Nov 1, 1993·Applied and Environmental Microbiology·C H Nakatsu, R C Wyndham
Jan 18, 2003·Journal of Microbiological Methods·Michael D BraidChristopher L Kitts
Apr 1, 1992·Applied and Environmental Microbiology·A A Khan, S K Walia
Mar 1, 1991·Applied and Environmental Microbiology·Y L TsaiB H Olson
Jun 1, 1991·Applied and Environmental Microbiology·Paul A RochelleBetty H Olson
Apr 1, 1991·Applied and Environmental Microbiology·Y L Tsai, B H Olson
Feb 1, 1993·Applied and Environmental Microbiology·L G LeffL J Shimkets
Dec 1, 1992·Microbiological Reviews·J R van der MeerA J Zehnder
Jun 1, 1994·Applied and Environmental Microbiology·W H JeffreyR Von Haven

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioremediation (ASM)

Bioremediation is the treatment and removal of harmful pollutants or contaminants through the use of microorganisms. Discover the latest research here.