PMID: 9435066Jan 22, 1998Paper

Construction and characterization of a 1,3-propanediol operon

Applied and Environmental Microbiology
F A SkralyD C Cameron

Abstract

The genes for the production of 1,3-propanediol (1,3-PD) in Klebsiella pneumoniae, dhaB, which encodes glycerol dehydratase, and dhaT, which encodes 1,3-PD oxidoreductase, are naturally under the control of two different promoters and are transcribed in different directions. These genes were reconfigured into an operon containing dhaB followed by dhaT under the control of a single promoter. The operon contains unique restriction sites to facilitate replacement of the promoter and other modifications. In a fed-batch cofermentation of glycerol and glucose. Escherichia coli containing the operon consumed 9.3 g of glycerol per liter and produced 6.3 g of 1,3-PD per liter. The fermentation had two distinct phases. In the first phase, significant cell growth occurred and the products were mainly 1,3-PD and acetate. In the second phase, very little growth occurred and the main products were 1,3-PD and pyruvate. The first enzyme in the 1,3-PD pathway, glycerol dehydratase, requires coenzyme B12, which must be provided in E. coli fermentations. However, the amount of coenzyme B12 needed was quite small, with 10 nM sufficient for good 1,3-PD production in batch cofermentations. 1,3-PD is a useful intermediate in the production of polyest...Continue Reading

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Citations

Mar 12, 2016·Biotechnology for Biofuels·Wei JiangBaishan Fang
Jan 14, 2009·Applied and Environmental Microbiology·Xueming TangWei Shen
Oct 25, 2012·Applied Microbiology and Biotechnology·André PickVolker Sieber
Apr 10, 2010·Journal of Industrial Microbiology & Biotechnology·Yu-Tze HorngPo-Chi Soo
Feb 10, 2010·Applied Microbiology and Biotechnology·James M Clomburg, Ramon Gonzalez
Oct 15, 2011·Critical Reviews in Biotechnology·E Celińska
Dec 24, 2015·Applied Microbiology and Biotechnology·Jie LinHeng Gong
Mar 23, 2013·Biotechnology Journal·Kai ZhuangMarkus J Herrgård
Jan 6, 2009·Journal of Applied Microbiology·Z RaoX Wang
Aug 1, 2018·Biotechnology and Applied Biochemistry·Lijuan ZhangHeng Gong
Dec 24, 2018·Applied and Environmental Microbiology·Mamou DialloAna M López-Contreras
Apr 14, 2016·Journal of Bioscience and Bioengineering·Mitsuru MatsubaraMichihiko Kataoka
Jan 11, 2001·Biochimica Et Biophysica Acta·G ChotaniK Sanford
Jul 6, 2010·New Biotechnology·Anand HiremathVidhya Rangaswamy
Aug 12, 2009·Biotechnology Advances·R K SaxenaJasmine Isar
Apr 7, 2010·Biotechnology Advances·E Celińska

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