Construction and characterization of a bacterial artificial chromosome library of thermo-sensitive genic male-sterile rice 5460S

Science in China. Series C, Life Sciences
F QiuBin Wang

Abstract

In order to develop a detailed physical map of the thermo-sensitive genic male-sterile (TGMS) gene-encompassing region and finally clone the TGMS gene, a high-quality rice bacterial artificial chromosome (BAC) library from TGMS rice 5460S was constructed. The method of constructing BAC library was examined and optimized. The 5460S library consists of 19 584 BAC clones with an average insert size of 110 kb, which represents about 5 times rice haploid genome equivalents. Rice inserts of up to 140 kb and 250 kb were isolated and appeared stable after 100 generations of serial growth. Hybridization of BAC clones with mitochondrial and chloroplastic genes as probes demonstrated that this library has no organellar contamination. The 5460S library was screened with 3 molecular markers linked to tms 1 gene as probes and at least 1 BAC clone was identified with each probe. The insert ends of positive clones were successfully isolated using thermal asymmetric interlaced PCR (TAIL-PCR) technique.

References

Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·H ShizuyaM Simon
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G A SilvermanS J Korsmeyer
Jun 11, 1995·Nucleic Acids Research·Y ShengB Birren
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·J JiangD C Ward
Sep 8, 2011·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Hongyun WangGary J Muehlbauer
Nov 1, 1995·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·B WangH T Nguyen

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Citations

Jan 7, 2014·Plant Science : an International Journal of Experimental Plant Biology·Quan JiangYan-Jun Dong

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