Construction and characterization of a live attenuated vaccine candidate against Shigella dysenteriae type 1.

Infection and Immunity
S R KleeC A Guzmán

Abstract

Vaccine candidates against Shigella dysenteriae type 1, which is associated with the most severe cases of bacillary dysentery, were constructed. The rfp and rfb gene clusters, which code for S. dysenteriae 1 O antigen biosynthesis, were randomly integrated into either the chromosome or the virulence plasmid of the rough attenuated Shigella flexneri aroD strain SFL124-27 with a minitransposon carrying an arsenite resistance selection marker. The recombinant clones efficiently expressed the recombinant O antigen, exhibited a normal growth pattern, were able to invade and survive within eukaryotic cells to the same extent as the parental strain, and expressed the recombinant antigen within invaded cells. A clone was selected as the vaccine candidate, which was demonstrated to be immunogenic and safe in animal models, leading to 47% full protection and 53% partial protection against challenge with the wild-type strain.

References

Nov 1, 1991·Infection and Immunity·A B HartmanK H Eckels
Jun 1, 1990·Microbial Pathogenesis·A A LindbergB A Stocker
Mar 1, 1990·The Journal of Infectious Diseases·M L BennishM Struelens
Jan 1, 1989·The American Journal of Tropical Medicine and Hygiene·D N TaylorA A Lindberg
Jun 1, 1989·The Journal of Infectious Diseases·H L DuPontS B Formal
Sep 1, 1989·The Journal of Infectious Diseases·E L LopezM Vasquez
Jan 1, 1982·Analytical Biochemistry·C M Tsai, C E Frasch
May 1, 1982·The Medical Clinics of North America·M M Levine
Mar 1, 1982·Infection and Immunity·P J SansonettiS B Formal
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S M HallingN Kleckner
Mar 1, 1981·Journal of Bacteriology·C I Kado, S T Liu
Dec 1, 1993·Infection and Immunity·M B Goldberg, P J Sansonetti

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Citations

Jun 30, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·G DouganC Simmons
Oct 19, 1999·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·A F TrofaM Yoshikawa
Jun 30, 2010·BMC Complementary and Alternative Medicine·Tannaz BirdiNoshir Antia
Jul 18, 2006·Journal of Zhejiang University. Science. B·S BrijeshT J Birdi
Mar 23, 2000·FEMS Immunology and Medical Microbiology·E Medina, C A Guzmán
Feb 27, 2001·Biomolecular Engineering·B Drabner, C A Guzmán
Nov 8, 2003·Journal of Clinical Microbiology·Kaisar A TalukderDavid A Sack

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